本研究將蠶絲蛋白與幾丁聚醣，運用靜電紡絲加工技術來製備複合奈米纖維膜，並浸泡中藥材紫草素溶液以應用於創傷敷材。其中探討蠶絲蛋白與幾丁聚醣其混摻溶液之不同比例對成絲之影響，以製作最佳化的蠶絲蛋白/幾丁聚醣複合奈米纖維膜，並測試纖維膜的水氣穿透率、含水能力及浸泡過中藥材紫草素後的抗氧化能力。另外並測試此膜對人類纖維母細胞L929的增生功能和毒性，以評估含紫草素纖維膜的生物相容性。並用人類黑色瘤细胞A375-S2之凋亡的現象評估其細胞增生性。此電紡纖維膜之抑菌性則使用革蘭氏陰性菌--大桿菌(Escherichia coli JM109, E. coli)及革蘭氏陽性菌--金黃色葡萄球菌(Staphylococcus aureus, S. aureus) (BCRC 10777)來測試。實驗結果顯示，裝載紫草素之蠶絲蛋白/幾丁聚醣電紡纖維膜具有應用於組織工程的創傷敷材之潛力。

In the study, silk fibroin (SF) and chitosan (CS) mat was prepared by electrospinning technique, followed by immersing in shikonin solution to evaluate the feasibility of these mats for wound dressing. SF/CS nanofibers provided a better level of equilibrium water content (EWC) and water vapor transmission rate (WVTR). Antioxidant activity of shikonin-loaded SF/CS mats was measured by the DPPH assay. Cytotoxicity of shikonin-loaded SF/CS mat was evaluated using L929 and A375-S2 fibroblasts through the MTT assay. The antibacterial activity of shikonin-loaded SF/CS mat was evaluated against E coli and S. aureus. Through characterization of physical properties, stability testing, and biocompatibility, the shikonin-loaded SF/CS mat exhibited potential for wound dressing applications.

第六章 文獻參考
1. Yi YD, Chang IM, An overview of Traditional Chinese Herbal formulae and a proposal of a new code system for expressing the formula titles. Advance Access Publication, 4: p. 125-132, 2004.
2. Winterstein P, Storrs CM, Herbal supplements: considerations for the athletic trainer. Journal of Athletic Training, 36 (4):p. 425-432, 2001.
3. Cooley JF, Apparatus for Electrically Dispersing Fluids. (US patent NO.692, 631), 1902.
4. Morton WJ, Method of dispersing fluids. US Patent, Serial No. 705,691, July. 29, 1902.
5. Zeleny J, The electrical discharge from liquid points, and a hydrostatic method of measuring the electric intensity at their surfaces. Phys. Rev.3, p. 69–91, 1914.
6. Taylor G, Disintegration of Water Drops in an Electric Field. Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences, 280:p.383-397, July 28, 1964.
7. Taylor G, The Force Exerted by an Electric Field on a Long Cylindrical Conductor. Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences, vol. 291, p.145-158, April 5, 1966.
8. Taylor G, Electrically Driven Jets. Proceedings of the Royal Society of London, Series A, Mathematical and Physical Sciences, 313: No. 1515, p. 453-475, Dec. 1969.
9. Simons HL, Process and apparatus for producing patterned non-woven fabrics. United States Patent 3280229, 1966.
10. Doshi J, Reneker DH, Electrospinning process and applications of electrospun fibers. J Electrostatics. 35(2 - 3): p. 151 - 60. 1995 Aug
11. Carnell LS, Siochi EJ, Holloway NM, Clark RL, Aligned mats from electrospun single fibers. Macromolecules, 41:p. 5345-5349, 2008.
12. Merritt SR, Exner AA, Lee ZH, Horst A. Electrospinning and Imaging. Advanced Engineering Materials, 14(5):B266–B278. Retrieved 1 December 2011.
13. Tan SH, Inai R, Kotaki M, Ramakrishna S. Systematic parameter study for ultra-fine fiber fabrication via electrospinning process. Polymer, 46: p.6128-6134, 2005.
14. 吳孟修, Electrospun core-shell fibers of alginate and chitosan for wound dressing碩士,材料科學與工程系,國立台灣科技大學,台北市,2012.
15. Nie H-R, He A-H, Zheng J-F, Xu S-S, Li J-X, and Han C-C, Effects of Chain Conformation and Entanglement on the Electrospinning of Pure Alginate. Biomacromolecules, 9: p. 1362-1365, 2008.
16. Shenoy SL, Bates WD, Frisch HL, Wnek GE, Role of chain entanglements on fiber formation during electrospinning of polymer solutions: good solvent, non-specific polymer-polymer interaction limit. Polymer, 46:p. 3372-3384, 2005.
17. Gupta P, Elkins C, Long TE,. Wilkes GL, Electrospinning of linear homopolymers of poly(methyl methacrylate): exploring relationships between fiber formation, viscosity, molecular weight and concentration in a good solvent. Polymer, vol. 46, pp. 4799-4810, 2005.
18. Wang C, Chien H-S, Hsu C-H, Wang Y-C, Wang C-T, Lu H-A, Electrospinning of polyacrylonitrile solutions at elevated temperatures, Macromolecules, 40:p. 7973-7983, 2007.
19. Sill TJ, von Recum HA, Electrospinning: Applications in drug delivery and tissue engineering. Biomaterials, 29: p. 1989-2006, 2008.
20. Bhardwaj N, Kundu SC, Electrospinning: A fascinating fiber fabrication technique. Biotechnology Advances, 28:p. 325-347, 2010.
21. Veparia C, Kaplan DL, Silk as a biomaterial. Prog.Polym. Sci., 32:p. 991–1007,2007.
22. Yamada K, Tsuboi Y, Itaya A, AFM Observation of Silk Fibroin on Mica Substrates; Morphologies Reflecting the Secondary Structure. Thin Solid Films, 440:p. 207, 2003.
23. Ozturk N, Akgol S, Arısoy M, Denizli A ,Reversible adsorption of lipase on novel hydrophobic nanospheres. Separation and Purification Technology, 58:83-90, 2007.
24. Rathke TD, Hudson SM, Review of Chitin and Chitosan as Fiber and Film Formers. Journal of Macromolecular Science, Part C: Polymer Reviews, 34:p. 375-437, 1994.
25. Mi F-L, Shyu S-S, Wu Y-B, Lee S-T, Shyong J-Y, Huang R-N, Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing. Biomaterials, 22:p. 165-173, 2001.
26. Han J, Zhou ZY, Yin RX, Yang DZ, Nie J, Alginate-chitosan/hydroxyapatite polyelectrolyte complex porous scaffolds: Preparation and characterization. International Journal of Biological Macromolecules, 46:p. 199-205, 2010.
27. Ma Y-F, Feng Q-L, Alginate hydrogel-mediated crystallization of calcium carbonate. Journal of Solid State Chemistry, 184:p. 1008-1015, 2011.
28. Blois MS, Antioxidant Determinations by the Use of a Stable Free Radical. Nature 181:p.1199-1200 ,1958.
29. Vlietinck AJ, DeBruyne T, Apers S, Pieters LA, Plant-derived leading compounds for chemotherapy of human immunodeficiency virus(HIV) infection. Planta Medica.v.64:p.97-109, 1998.
30. Prabhakaran MP, Venugopal JR, Chyan TT, Hai LB, Chan CK, Lim AY, Ramakrishna S, Electrospun biocomposite nanofibrous scaffolds for neural tissue engineering. Tissue Engineering Part A, 14: p.1787-1797, 2008.
31. Lee JS, Kim JK, Chang YH, Preparation of collagen/poly(L-lactic acid) composite material for wound dressing. Macromolecular Research, 15: p. 205-210, (2007).
32. Queen D, Gaylor JDS, Evans JH, Courtney JM, Reid WH, The preclinical evaluation of the water vapour transmission rate through burn wound dressings. Biomaterials, 8:p. 367-371, 1987.
33. 吴 振、吴立军、田代真一、小野寺敏、池岛乔，紫草素诱导Hela细胞凋亡经过caspase激活的机制，中国药理学通报,2004,20(5)：p.540－544.
34. 吴 振、吴立军、田代真一、小野寺敏、池岛乔，紫草素诱导A375-S2 细胞凋亡的分子机制研究，中国药理学通报,2005,21(2)：p.202－205.