研究生: |
陳勁齊 Chin-Chi Chen |
---|---|
論文名稱: |
HEMA/PEGMA水膠之製備及其在隱形眼鏡材料之應用 Preparation of HEMA/PEGMA blend hydrogels for contact lens material |
指導教授: |
楊銘乾
Ming-Chien Yang |
口試委員: |
王大銘
Da-Ming Wang 蘇清淵 Ching-Yuan Su |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 材料科學與工程系 Department of Materials Science and Engineering |
論文出版年: | 2010 |
畢業學年度: | 98 |
語文別: | 中文 |
論文頁數: | 85 |
中文關鍵詞: | HEMA 、PEGMA 、抗蛋白 、水膠 、隱形眼鏡 |
外文關鍵詞: | HEMA, PEGMA, anti-protein, hydrogel, contact lens |
相關次數: | 點閱:652 下載:9 |
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本文以甲基丙烯酸-2-羥基乙酯(2-Hydroxylethyl methacrylate, HEMA)及親水性抗蛋白單體甲基丙烯酸聚乙二醇酯(poly(ethylene glycol) methacrylate, PEGMA)為單體,再加入diethoxy acetophenone (DEAP) 光起始劑以及交聯劑Tetraethyl Orthosilicate (TEOS),以UV起始法經由自由基共聚合製備HEMA-PEGMA水膠,以提高HEMA水膠之親水性以及增加其抗蛋白性。
根據實驗結果得知,HEMA-PEGMA水膠的含水率隨著PEGMA的含量增加而上升,而接觸角也因PEGMA的加入而降低,因而提高水膠的親水性。此外,氧穿透率(oxygen transmittance,Dk )也相對增加。相反地,其機械強力(mechanical strength)方面卻會隨著PEGMA的增加而有下降的趨勢。HEMA-PEGMA水膠的透光度在可見光範圍可達94%以上。而在蛋白質吸附試驗中,我們利用BCA 蛋白質測定方法來探討,結果顯示隨著PEGMA的含量增加,能夠明顯的降低蛋白質吸附。在細胞毒性方面,以ISO10993-5的判定上屬於0-1 級,所以其細胞毒性是通過標準的。針對這些特性,未來可以利用此水膠來作為隱形眼鏡及眼科材料之運用。
In this work, hydroxyethyl methacrylate (HEMA) was copolymerized with poly(ethylene glycol) methacrylate (PEGMA) into hydrogel via ultraviolet (UV) initiation with diethoxy acetophenone (DEAP) as the initiator and tetraethyl orthosilicate (TEOS) as the crossliking agent to increase hydrophilicity and anti-protein adsorption.
The results show that the increase in PEGMA content led to the decrease of water contact angle and the increase of water content (EWC) for the blend hydrogels. Moreover, the oxygen transmittance (Dk) increased with the increase of EWC. The mechanical strength of the hydrogel also decreased with the PEGMA content. The HEMA-PEGMA hydrogels exhibited high optical transmittance (over94%). When the PEGMA content reached 20%, the apparent protein adsorption amount decreased to about 75% of that of HEMA control. Thus the HEMA-PEGMA hydrogels exhibited the ability to resist protein adsorption. Furthermore, these hydrogel were non-cytotoxic through in vitro L929 fibroblasts proliferation assay. Overall results demonstrated that the HEMA-PEGMA hydrogels exhibited relatively high oxygen permeability, hydrophilicity, optical transparency, and anit-protein adsorbtion , therefore would be applicable as contact lens material.
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