本研究旨在開發具有病理切片與染劑濃度測試雙項功能的複合式微生物組織陣列自動化設備。並且針對生物檢體的鑽取準確性、鑽取位置數量規劃以及蠟塊封裝品質進行探討。研究的進行可分為設備規劃、影像疊合、鑽取位置與數量之指定以及最佳參數搜尋等四階段。首先是評估複合式機台的功能需求，進而構思設備之組成架構。此階段同時探討複合式功能之自動化流程概念，藉以釐清達成自動化的必要條件。其次是生物組織影像疊合方法的實踐。研究中利用基因演算法的尋優特性得到影像疊合的最佳平移與旋轉參數，藉以提升組織的鑽取準確性。第三階段論述鑽取數量與位置規劃方法。本研究嘗試以力平衡為基礎的元件放置方法，藉由持續調整元件間之彈簧常數而得到適當的鑽取數量與位置。最後的階段則是為了提升組織陣列蠟塊的封裝品質，因而利用田口式品質工程實驗方法取得較佳的封裝製程參數。由實作開發的機台進行實驗後發現，使用者可以完整地規劃生物檢體的鑽取數量與位置，而系統也可以準確的自動鑽取並得到品質良好的生物檢體。至於使用穩健化設計得到的製程參數，能夠有效的提升微生物組織陣列蠟塊的封裝品質。

This study aims to develop a multi-function automatic tissue microarrayer that incorporates functions accommodating both the pathological analysis and the staining reagent concentration tests. Furthermore, tissue alignment accuracy, tissue punching position and quantity planning, and tissue microarray block (TMA) package quality are also discussed. This study contains four stages. First, through evaluating functional requirements including both pathological analysis and staining concentration tests, the possible structure to design the apparatus is considered based on these concepts. In addition, a concept of automatic flow is also discussed in order to find out the necessary condition for achieving multi-function automation. A genetic algorithm (GA) is then performed to obtain the optimal translation and rotation parameters for image alignment to improve alignment accuracy. Next, a punching position and quantity planning is discussed using force-directed placement algorithm to continually modify the spring constant between components and establish the appropriate punching position and quantity. Finally, to improve the TMA block package quality, Taguchi method is employed for TMA process to obtain the optimal assembly process parameter. Experimental results revealed that self-developed tissue microarrayer can not only efficiently enhance tissue alignment accuracy but also completely plan tissue punching position and quantity. As for TMA process, the robust parameters can obviously improve the package quality of TMA block.

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