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研究生： NUR HASANAH
NUR HASANAH
論文名稱： 具物理性質及生物相容性之TRIS-NVP-PEGMA-MAA 複合水凝膠於眼部之應用
Physical properties and biocompatibility of TRIS-NVP-PEGMA-MAA hydrogel for ophthalmic application2019
指導教授： 楊銘乾
MING-CHIEN YANG
口試委員: 劉定宇
TING-YU LIU
鄭詠馨
Yung-Hsin Cheng
學位類別： 碩士
Master
系所名稱： 工程學院 - 材料科學與工程系
Department of Materials Science and Engineering
論文出版年： 2019
畢業學年度： 107
語文別： 英文
論文頁數： 62
中文關鍵詞： TRIS 、NVP 、PEGMA 、MAA 、矽水凝膠 、隱形眼鏡
外文關鍵詞： TRIS, NVP, PEGMA, MAA, silicone hydrogel, contact lens
相關次數： 點閱：447  下載：22 
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  •  上一筆

    • 本論文經由光起始自由基聚合方法合成有機矽水凝膠，其單體為3- [三（三甲基甲矽烷氧基）甲矽烷基]丙基甲基丙烯酸酯(3-[tris(trimethylsiloxy)silyl]propyl methacrylate,TRIS)，N-乙烯基吡咯烷酮(N-vinylpyrrolidone,NVP)，甲基丙烯酸(methacrylic acid, MAA)，以及聚（乙二醇）甲基丙烯酸酯(poly(ethylene glycol) methacrylate, PEGMA)，交聯劑為乙二醇二甲基丙烯酸酯(ethylene glycol dimethacrylate, EGDMA)，並以2-羥基-2-甲基苯丙酮(2-hydroxy-2-methylpropiophenone,PI-1173)為光起始劑，1-己醇(1-hexanol)作為溶劑。隨著PEGMA含量之增加，凝膠之合成率由70%逐步下降，平衡含水率(EWC)上升，拉伸強度及楊氏模數增加。由於PEGMA的抗垢特性，溶菌酶及人血清蛋白(HSA)的吸附量極低。此外，根據體外L929纖維母細胞之測定，證實這些矽水凝膠無細胞毒性。根據以上實驗結果，顯示TRIS-NVP-PEGMA-MAA共聚物由於高含水量，優異的光學透明度，低蛋白質吸收和生物相容性，成為隱形眼鏡的良好候選材料。

    A silicone hydrogel was synthesized through photoinitiated free radical polymerization process using 3-[tris(trimethylsiloxy)silyl]propyl methacrylate (TRIS), N-vinylpyrrolidone (NVP), poly(ethylene glycol) methacrylate (PEGMA), methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA) as cross-linker, 2-hydroxy-2-methylpropiophenone (PI-1173) as photoinitiator, and 1-hexanol as solvent. The gel fraction values are around 70 % and decrease with the increase of PEGMA content in the formulation due to the poor copolymerization of methacrylate functional monomers and NVP. Increase the content of PEGMA led the higher EWC. This is due to the changes in hydrophilic and hydrophobic monomer ratio in the formula. The tensile strength and Young’s modulus of hydrogels were higher with the addition of PEGMA. The adsorbed amount of lysozyme and HSA was very small due to nonfouling properties of hydrogels. In addition, these silicone hydrogels were confirmed non-cytotoxic according to an in-vitro L929 fibroblast assay. In summary, the results illustrated that TRIS-NVP-PEGMA-MAA copolymer was a good candidate for contact lens material due to the high water content, excellent optical transparency, low protein adsorption, and biocompatibility.

    ABSTRACT i 摘要 ii ACKNOWLEDGEMENTS iii List of Content iv List of Figures vii List of Tables viii Chapter 1 Introduction 1 1.1 Background of study 1 1.2 Motivation and the objectives of study 2 Chapter 2 Literature Review 4 2.1 Hydrogels 4 2.2 Hydrogel classification 5 2.2.1 Chemical hydrogel 5 2.2.2 Physical Hydrogel 6 2.3 Cross-linking in hydrogel 6 2.4 Contact lens 8 2.5 Classification of Contact Lenses 9 2.5.1 Rigid contact lens 9 2.5.2 Soft Contact Lens 9 2.6 Properties of soft Contact lens material 10 2.6.1 Water content 11 2.6.2 Surface wetting 12 2.6.3 Oxygen permeability 13 2.6.4 Mechanical Properties 15 2.6.5 Surface roughness 16 2.6.6 Biocompatibility 16 2.7 Materials introduction 18 2.7.1 3-Methacryloyloxypropyltris(trimethylsilyloxy)silane(TRIS) 18 2.7.2 N-vinyl pyrrolidone (NVP) 19 2.7.3 Poly(ethylene glycol) methacrylate (PEGMA) 20 2.7.4 Methacrylic acid (MAA) 20 2.7.5 Ethylene glycol dimethacrylate (EGDMA) 21 Chapter 3 Materials and Methods 22 3.1 Experimental process 22 3.2 Experimental Principle 23 3.3 Materials 23 3.4 Analyzers 24 3.5 Experimental Steps 24 3.6 Physical Characterization Testing 25 3.6.1 Gel fraction 25 3.6.2 Equilibrium water content 25 3.6.3 Contact Angle Measurement 26 3.6.4 Light Transmittance 26 3.6.5 Mechanical strength 27 3.7 Biocompatibility testing 28 3.7.1 Protein Adsorption 28 3.7.2 Cell toxicity 29 Chapter 4 Result and discussion 31 4.1 Gel fraction 31 4.2 Equilibrium water content (EWC) 32 4.3 Contact Angle 34 4.4 Light Transmittance 35 4.5 Mechanical Strength 36 4.6 In vitro cytotoxicity 39 4.7 Protein adsorption 41 4.8 Preliminary comparison with conventional contact lens 42 References 47

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