Bacteriorhodopsin (bR)為Halobacterium salinarium嗜鹽菌紫色細胞膜上的獨一光驅動質子泵之光能轉換蛋白。本文分別利用polyacrylamide膠體及streptavidin/biotin兩套固定化系統，將bR蛋白包埋固定及方向性固定化於ITO導電玻璃上，以作為光電化學系統之工作電極。利用微電流儀以及bio-AFM、X射線光電子能譜儀(XPS)和小角度X光散射儀(SAXS)，分別進行光電流訊號檢測及薄膜表面分析。結果證明bR複合薄膜具有良好之透光性，且bR蛋白保持了原有的生物活性和光學性質;另外在週期性光照射下，發現於弱鹼環境下具有最佳之光電信號響應;而以streptavidin/biotin系統所固定之薄膜兼具了方向性固定化之優點，此研究對於未來生物光電元件之開發提供絕佳的固定化方法。

Bacteriorhodopsin (bR) is a membrane protein of photon-driven proton pump from Halobacterium salinarium. This research employed the techniques of polyacrylamide-gel entrapment and orientational immobilization based on streptavidin-biotin interaction in preparing two different photoelectric bR biochips, respectively, which was each used as the working electrode of an electrochemical system. The photoelectric response and surface analysis of bR biochips were analyzed using picoammeter、bio-AFM、XPS and SAXS, respectively. The results showed that the prepared bR complex films exhibited high transparence, within which bR kept its biologic activities and photo response. With periodic illumination, the chips displayed an optimal photoelectric response in a weakly alkaline condition. In addition, the immobilization via streptavidin-biotin interaction offers the advantage of directional orientation, which is highly critical for the development of bio-photoelectric devices.

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