膠原蛋白/不織布樣品製備與吸放膠原蛋白溶液性能研究

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研究生：	李茹娟 Ru-chuan Lee
論文名稱：	膠原蛋白/不織布樣品製備與吸放膠原蛋白溶液性能研究 Preparation and investigation of the absorption and desorption properties of collagen solution of collagen/nonwoven specimens
指導教授：	葉正濤 Jen-taut Yeh
口試委員:	陳耿明 Keng-ming Chen 張豐志 Feng-chih Chang 芮祥鵬 Rwei-shan Paio 陳幹男 Kan-nan Chen
學位類別：	碩士 Master
系所名稱：	工程學院 - 材料科學與工程系 Department of Materials Science and Engineering
論文出版年：	2006
畢業學年度：	94
語文別：	中文
論文頁數：	88
中文關鍵詞：	膠原蛋白、不織布、植絨
外文關鍵詞：	collagen, nonwoven, flocking
相關次數：	點閱：210 下載：0
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本研究主要探討牛第三型膠原蛋白(Bovine Type III collagen)/植絨不織布(MPPFN-X/Y)樣品製備與膠原蛋白溶液吸收性能，並討論最適化植絨不織布製程。將牛皮以鹼及酸溶液進行提取及純化膠原蛋白溶液，再將膠原蛋白溶液與不同長度及細度之Nylon植絨不織布(MPPFN-X/Y)進行吸濕測試。結果發現，經選用適當之MPPFN-X/Y樣品可以得到最佳吸放膠原蛋白溶液性能。在此最適化加工條件下之MPPFN-X/Y樣品，其吸收性能優於一般水針不織布樣品。為瞭解膠原蛋白溶液/MPPFN-X/Y樣品吸放性能的關係，膠原蛋白電泳分析、凝膠層析分析及吸放性能性質均在本文中討論。

In this study, preparation and investigation of the absorption and desorption properties of bovine type Ⅲ collagen / flocking nonwovens (MPPFN-X/Y) is reported, the collagen solution was extracted by using base and acid solution from bovine skin, and processing wicking test for different length and denier of flocking nonwovens, moreover, the optimization of the MPPFN-X/Y sample better than spundlace. To realize these interesting absorption and desorption properties, the SDS-PAGE(sodum dodecylsulfatepoly acrylamide gel electrophoresis), GPC(gel permeation chromatography), wicking test have been every discussed in this paper.

論文摘要.....................................................................................................I
ABSTRACT ..............................................................................................II
誌謝..........................................................................................................III
目錄..........................................................................................................IV
圖表索引..................................................................................................VI
第一章 前言..............................................................................................1
第二章 文獻回顧......................................................................................3
2.1  膠原蛋白簡介...................................................................................3
2.2  膠原蛋白之分子及纖維結構……………………………………...6
2.3  膠原蛋白之純化及重組………………………………………….11
2.4  皮膚與老化(aging)………………………………………………..15
2.5  膠原蛋白與相關疾病及研究…………….....................................18
2.5.1 膠原蛋白在生醫上之應用…................................................19
2.6  不織布的簡介…………………..………………………………...22
第三章 實驗部份………………………………………………………26
3.1  藥品.................................................................................................26
3.2  儀器設備.........................................................................................28
3.3  膠原蛋白之製備……………….....................................................30
3.3.1 由牛皮組織中萃取純化第三型膠原蛋白…………............30
3.3.2 膠原蛋白結構與分子量測定………………………………32
3.3.3 膠原蛋白濃度分析……………………...............................37
3.4  面膜不織布樣品製備…………….................................................40
3.4.1 面膜不織布對膠原蛋白溶液吸收性能分析........................45
3.4.2 面膜不織布樣品表面形態分析............................................46
第四章 結果與討論................................................................................47
4.1  膠原蛋白的萃取與純化…………….............................................47
4.1.1 膠原蛋白結構與分子量分析................................................47
4.1.2 膠原蛋白溶液濃度分析........................................................56
4.2  面膜不織布對膠原白溶液吸收性能分析…………….................59
4.2.1不同絨毛長度樣品.................................................................59
4.2.2不同植絨纖維細度樣品..........................................................61
4.2.3水針與植絨不織布面膜樣品膠原蛋白溶液吸收性能..........63
4.2.4絨毛立體結構表面分析….....................................................65
第五章 結 論..........................................................................................70
參考文獻..................................................................................................72
作者簡介..................................................................................................77

圖表索引
一、	圖索引
圖2.1-1 七類不同分子結構之膠原蛋白	4
圖2.2-1 膠原蛋白分子之三股螺旋結構示意圖	8
圖2.2-2 膠原蛋白纖維組成示意圖	9
圖2.2-3 膠原蛋白纖維之穿透式電子顯微鏡照片	10
圖2.3-1 膠原蛋白分子重組現象	13
圖3.3.1-1萃取純化第三型膠原蛋白實驗流程	31
圖3.4-1 植絨工程流程圖...................................................................... 42
圖3.4-2 (a)植絨箱、(b)方型框架、(c)固定框架邊條、(d)電極板、(e)高壓正電供應器及(f)地線等六種元件所組成之植絨箱裝置圖..............................................................................................43
圖4.1.1-1  Lane 1蛋白質標準液、Lane 2 , 3 , 4,樣品為含有10μg、15μg及20μg牛第三型膠原蛋白樣品之SDS-PAGE結果圖譜..........................................................................................49
圖4.1.1-2  (a) Blue dextran、(b) PEO 800k、(c)PEO 400k及(d1) monomer BSA和(d2) dimer BSA等標準品GPC分析後之結果圖................................................................................51
圖4.1.1-3  (a) Blue dextran、(b) PEO 800k、(c)PEO 400k及(d) BSA等不同分子量標準品之標準曲線(R2＝0.9047).................52
圖4.1.1-4 第三型膠原蛋白溶液之GPC分析圖...................................55
圖4.1.2-1 氫脯胺酸標準液之檢量線....................................................57
圖4.2.1-1 (●) MPPFN-1.5 / 0.5、(●) MPPFN-1.5 / 1、(●) MPPFN-1.5 / 1.5及(●)
 MPPFN-1.5 / 2 不同絨毛長度面膜不織布植絨密度與吸收膠原蛋白重量作圖........................................................................60
圖4.2.2-1 (●) MPPFN-1 / 1.5、(●) MPPFN-3 / 1.5及(●) MPPFN-7 / 1.5不同絨毛細度面膜不織布植絨密度與吸收膠原蛋白重量作圖............................................................................................62
圖4.2.3-1 (a)水針樣品、(b)MPPFN-1.5 / 0.5、(c) MPPFN-1.5 / 1、(d) MPPFN-1.5 / 1.5、(e) MPPFN-1.5 / 2與(f)MPPFN-1/ 1.5、(g) MPPFN-3 / 1.5、(h) MPPFN-7 / 1.5植絨不織布樣品之最高膠原蛋白吸收重量(mg/cm2)比較圖............................................................................................64

圖4.2.4-1 (a)MPPFN-1.5/0.5(植絨密度45000根/cm2)、(b)MPPFN-1.5/1(植絨密度20000根/cm2)、(c)MPPFN-1.5/1.5(植絨密度12000根/cm2)、(d)MPPFN-1.5/2面膜不織布(植絨密度8000根/cm2)及(e)MPPFN-1.5/0.5、(f)MPPFN-1.5/1、(g)MPPFN-1.5/1.5、(h)MPPFN-1.5/2 面膜不織布吸收膠原蛋白溶液後之絨毛表面型態……...66
圖4.2.4-2  (a)MPPFN-1.5/0.5、(b)MPPFN-1.5/1、(c)MPPFN-1.5/1.5、(d)MPPFN-1.5/2面膜不織布及(e)MPPFN-1.5/0.5、(f)MPPFN-1.5/1、(g)MPPFN-1.5/1.5、(h)MPPFN-1.5/2 面膜不織布吸膠原蛋白溶液後之絨毛表面型態...........................................................................................68

二、	表索引
表2.1-1 膠原蛋白的類型........................................................................5
表2.3-1 不同種類膠原蛋白沉澱析出所需之鹽濃度...........................14
表2.4-1 皮膚因慢性老化產生的現象...................................................17
表2.4-2 皮膚因光老化產生的現象.......................................................17
表2.5-1 膠原蛋白在醫學上的應用.......................................................18
表2.5.1-1 膠原蛋白做為生醫材料之優缺點........................................20
表2.5.1-2 商業化膠原蛋白產品及生產公司........................................21
表2.6-1 1985-2005年高科技紡織品分類消費統計表..........................23
表2.6-2 Spunlace process優缺點............................................................25
表3.3.2-1 6%分離膠體溶液及4%集焦膠體溶液配方..........................34
表3.3.2-2 電泳緩衝液配方....................................................................34
表3.3.2-3 試樣緩衝液配方....................................................................35
表3.3.2-4 CBR染液配方........................................................................35
表3.3.2-5 脫色液配方........................................................................35
表3.3.2-6 管柱緩衝液配方...................................................................36
表3.3.3-1 Chloramine-T試劑配方......................................................39
表3.3.3-2 Ehrlich’ s試劑配方.............................................................39
表3.4.-1 植絨不織布規格總表.................................................44
表4.1.2-1 氫脯胺酸標準液檢量線........................................................57
表4.1.2-2 第三型膠原蛋白溶液實際濃度分析結果............................58
表4.2.4-1 面膜不織布吸收膠原蛋白前後傾斜角度關係整理表.........69

                                

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