研究生: |
蔡毅霖 Yi-Lin Tsai |
---|---|
論文名稱: |
含ε-聚賴氨酸-AOT複合物之電紡聚丙烯腈纖維 製備及其抗菌研究 Electrospun Polyacrylonitrile nanofibrous fiber containing water insoluble ε-Polylysine-AOT complex and its application for Antimicrobial |
指導教授: |
李振綱
Cheng-Kang Lee |
口試委員: |
蔡伸隆
Shen-Long Tsai 王鐘毅 Chung-Yih Wang |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2019 |
畢業學年度: | 107 |
語文別: | 中文 |
論文頁數: | 107 |
中文關鍵詞: | 靜電紡絲 、抗菌 、幾丁聚醣 、聚賴氨酸 |
外文關鍵詞: | electrospinning, antimicrobial, chitosan, ε-Polylysine |
相關次數: | 點閱:385 下載:1 |
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現今生醫材料中已發展出許多具抗菌能力的敷料,目的是對傷口處之細菌進行抑制以防止感染。例如含奈米銀的敷材雖能快速產生殺菌作用,但奈米銀顆粒也會隨擴散而消耗;本論文之研究方向為製備不具擴散能力的兩類抗菌材料並經CFU試驗檢測抗菌能力:第一類是製備帶胺基的幾丁聚醣與聚乙烯醇混合之電紡絲膜(PVA/CS),其對大腸桿菌有優異的抗菌效果;透過與戊二醛及多巴胺進行表面修飾後可將酵素lysostaphin固定化於材料,其對金黃色葡萄球菌有優異的抗菌能力。第二類材料是利用水溶之抗菌胜肽ε-Polylysine與陰離子表面活性劑AOT結合,製備不溶水之複合物(AP)。以二甲基甲醯胺為溶劑溶解AP可與溶於二甲基甲醯胺之聚丙烯腈溶液混合,經電紡絲得具抗菌功能之非水溶的耐水性電紡絲膜(PAN/AP)。此材料以CFU法檢測抗菌效果發現,對大腸桿菌的抗菌能力可達與聚乙烯醇電紡絲膜接近的80 %的抗菌效果,而對金黃色葡萄球菌的抗菌能力則會比固定化酵素lysostaphin的75 %更好。此材料除了用CFU試驗檢測抗菌效果,也使用濁度試驗檢測確認金黃色葡萄球菌培養後濁度下降情形;接觸式殺菌試驗也展現材料對金黃色葡萄球菌優異的抗菌能力,顯示其對大腸桿菌以及金黃色葡萄球菌皆能作用。
Antimicrobial electrospun nanofibrous mats were prepared based on two kinds of biopolymers. Chitosan and hydrophobically modified chitosan (HMCS) are known for their antimicrobial activity, however, they can not be easily electrospun into nanofibers. Aqueous solution of polyvinylalcohol (PVA) was found to be easily electrospun into nanofibrous mat. By electrospinning chitosan (CS) mixed PVA solution (6 wt% PVA+ 1wt% CS) a nice nanofibrous mat (averaged fiber diameter ca 250 nm) could be obtained. The mat demonstrated very good antibacterial results against Escherichia coli. Epsilon-polylysine (PL), an antimicrobial agent has been approved to use in food industry, can easily form a water insoluble complex with anionic surfactant dioctyl sulfosuccinate (AOT). The AOT-PL (AP) complex is well dissolved in dimethylformamide and can be co-electrospun with polyacrylonitrile (PAN). The diameter of obtained electrospun fiber increases with the addition of AP from 800 nm for 33 wt% AP to 2.5 um for 67 wt% AP. The obtained AP mat is water durable. The antimicrobial activity test shows that bacteria could only be contact-killed by the AP mat. It has nearly 80 % antibacterial effect against Escherichia coli, and more than 75 % antibacterial activity against Staphylococcus aureus.
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