研究生: |
陳俊宏 Chun-hung Chen |
---|---|
論文名稱: |
二性次微米高分子粒子與蛋白質之間的交互作用 Interactions between Amphoteric Submicron Polymer Particles and Proteins |
指導教授: |
陳崇賢
Chorng-shyan Chern |
口試委員: |
林析右
Shi-yow Lin 李振綱 Cheng-kang Lee |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2005 |
畢業學年度: | 93 |
語文別: | 中文 |
論文頁數: | 74 |
中文關鍵詞: | 二性次微米高分子 、蛋白質 |
外文關鍵詞: | Amphoteric Submicron, Proteins, Polymer Particles |
相關次數: | 點閱:325 下載:2 |
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本研究以甲基丙烯酸甲酯(MMA)為疏水性單體、甲基丙烯酸(MAA)為親水性單體,V-50為陽離子起始劑,先以批次無乳化聚合反應合成種子乳漿粒子,再以半批次無乳化聚合反應合成乳漿粒子。這些次微米粒子的表面同時具有胺基及羧基,因此具有pH敏感性,在低pH值的環境下由於胺基團離子化使得粒子表面帶正電;在高pH值的環境下由於羧基團離子化使得粒子表面帶負電。
藉由兩性乳漿粒子分別與BSA、Pepsin、Lysozyme進行恆溫吸附以觀察膠體粒子濁度、粒徑及表面電性之變化,發現隨著不同的pH值環境下,乳漿粒子與三種蛋白質之交互作用也隨之改變,當兩性乳漿粒子與蛋白質帶有相反的異性電荷時,在電性中和下形成一個自發的吸附過程會造成凝聚的現象,是處於較不安定的膠體系統;當兩性乳漿粒子與蛋白質帶有同性的電荷時,會產生靜電排斥力使得蛋白質較不易吸附在粒子的表面,因此顯示具有較安定的膠體系統。
Amphoteric latex particles were prepared by the surfactant-free emulsion polymerization, with methyl methacrylate (MMA) as the hydrophobic monomer, methacrylic acid (MAA) as the hydrophilic monomer, and V-50 as the cationic initiator. First, the seed latex particles were prepared by the batch surfactant-free emulsion polymerization. Second, the latex products were synthesized by the semibatch seeded surfactant-free emulsion polymerization. These submicron particle surfaces have amino groups and carboxyl groups at the same time. Therefore, they display pH-sensitiy. The particle surfaces have net positive charge, owing to the ionized amino groups at low pH. The particle surfaces have net negatively charge, owing to the ionized carboxyl groups at high pH.
Isothermal adsorption of BSA, pepsin or lysozyme on amphoteric latex particles were carried out. Adsorption of proteins onto the particles leads to change in the turbidity, particle size and zeta potential. When amphoteric latex particles and proteins have the opposite net charge, the self-promoting adsorption process may occur due to charge neutralization and this could lead to flocculation. In contrast, electrostatic repulsion forces between amphoteric latex particles and proteins with similar charges play an important role in the colloidal stability. This will then retard the adsorption of protein molecules onto the particle surfaces. Therefore, latex particles exhibit excellent colloidal stability.
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