研究生: |
郭于甄 Yu-Chen Kuo |
---|---|
論文名稱: |
以冷凍乾燥法製備生醫玻璃/幾丁聚醣複合支架與性質鑑定 Characterization and preparation of freeze dried-bioactive glass/chitosan composite scaffolds |
指導教授: |
施劭儒
Shao-Ju Shih |
口試委員: |
施劭儒
王丞浩 周育任 鄭詠馨 |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 材料科學與工程系 Department of Materials Science and Engineering |
論文出版年: | 2020 |
畢業學年度: | 108 |
語文別: | 中文 |
論文頁數: | 104 |
中文關鍵詞: | 生醫玻璃 、骨組織工程 、噴霧乾燥法 、冷凍乾燥法 |
外文關鍵詞: | bioactive glass, bone tissue engineering, spray drying, freeze drying |
相關次數: | 點閱:317 下載:11 |
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目前全球皆面臨高齡化社會的問題,當人的年齡超過三十歲,骨質密度就會開始下降,因罹患骨質疏鬆症而造成的骨折相當常見,為修復骨缺損,需使用骨填充材,但現今自體移植材料短缺,異體移植材料易造成疾病感染及排斥,因此人工合成的骨組織支架被列為考慮。
幾丁聚醣具有優異的生物相容性、生物降解性、抗菌性以及促進傷口癒合的能力,其降解產物為無毒、非致癌性,同時又可幫助細胞貼附、增殖與分化;而生醫玻璃無毒,且擁有良好的生物相容性,亦具有生物降解性及生物活性,常被用來當作骨填充材,治療骨缺陷,因此本研究選取這兩者材料來製備骨組織複合支架。
首先,利用噴霧乾燥法製備生醫玻璃與摻雜不同鍶濃度(2.5、5.0、7.5及10.0 mol%)之生醫玻璃,並透過掃描式電子顯微鏡、X光繞射儀、傅立葉轉換紅外線光譜儀分析其晶相結構、粉體顆粒形貌、元素組成以及鍶對體外生物活性的影響。接著,使用冷凍乾燥法製造生醫玻璃/幾丁聚醣之複合支架,並利用掃描式電子顯微鏡、萬能試驗機、微量盤分光光度計及X光繞射儀、分析其巨觀形貌、微觀結構、孔隙率、機械性質、膨潤度、降解行為、細胞存活率與體外生物活性。
At present, the world is facing the problem of an aging society. When people are over 30 years old, bone density will start to decrease. Bone fractures caused by osteoporosis are quite common. To repair bone defects, bone filling materials are needed. For now, there is a shortage of autograft materials, and allograft materials are likely to cause disease infection and rejection. Therefore, synthetic tissue scaffolds are considered.
Chitosan has good biocompatibility, biodegradability, antibacterial properties and the ability to accelerating wound healing. Its degradation products are non-toxic and non-carcinogenic. Also, it can enhance cell adhesion, proliferation and differentiation. Bioactive glass (BG) is non-toxic, has good biocompatibility, biodegradability and bioactivity. It is often used as a bone filling material to treat bone defects, so in this study, these two materials were chosen to fabricate bone tissue composite scaffolds.
The first step, spray drying method was used to prepare 0.0, 2.5, 5.0, 7.5 and 10.0 mol% strontium-doped BG. Then using scanning electron microscope (SEM), X-ray diffractometer (XRD) and Fourier-transform infrared spectrometer (FTIR) to analyze the phase composition, morphology and in vitro bioactivity of BG and Sr-BG, respectively. Next step, freeze drying method was used to fabricate the Sr-BG/chitosan composite scaffolds, and the macroscopic morphology, elemental composition, microstructure, porosity, mechanical properties, swelling ratio, degradation behavior, cell viability and in vitro bioactivity were characterized by SEM, universal testing machine, microplate spectrophotometer and , XRD.
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