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研究生： 中西昭博
Akihiro Nakanishi
論文名稱： 噴霧乾燥製造β-磷酸三鈣與生物活性玻璃複合粉末之特性分析
Fabrication and characterization of granulated β-tricalcium phosphate/bioactive glass composited powders by spray drying
指導教授： 施劭儒
Shao-Ju Shih
口試委員: 王丞浩
Chen-Hao Wang
森賀俊広
Toshihiro Moriga
游進陽
Chin-Yang Yu
鄒年棣
Nien-Ti Tsou
施劭儒
Shao-Ju Shih
學位類別： 博士
Doctor
系所名稱： 工程學院 - 材料科學與工程系
Department of Materials Science and Engineering
論文出版年： 2023
畢業學年度： 112
語文別： 英文
論文頁數： 105
中文關鍵詞： β-磷酸三鈣 、生物活性玻璃 、噴霧熱解 、噴霧乾燥 、造粒
外文關鍵詞： β-tricalcium phosphate, bioactive glass, spray pyrolysis, spray drying, granulation
相關次數： 點閱：68  下載：0 
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  •  上一筆

    • 骨病或骨缺損是嚴重影響人類生活的問題。骨移植是常見的移植組織之一，磷酸鈣因其化學成分與人體骨骼相似而得到廣泛的應用。β-磷酸三鈣(β-TCP)具有生物相容性和生物吸收性，適用於骨骼移植。然而，由於β-TCP在體液中的溶解性佳，使其降解速度過快。年輕人骨骼生長速度快，而老年人骨骼生長速度慢，因此需要個人化的骨骼移植。有報道指出，β-TCP和生物活性玻璃(BG)複合材料可以實現個人化骨骼移植。
    在這項研究中，β-TCP和生物活性玻璃（BG）的顆粒狀粉末是通過噴霧熱解和噴霧乾燥製造的。這些製造方法適用於大規模生產和合成球形顆粒。首先，β-TCP和BG的球形顆粒是通過噴霧熱解合成的。之後，這些粉末以不同比例的β-TCP/BG=100/0, 75/25, 50/50, 25/75, 0/100進行噴霧乾燥製粒。用XRD、FT-IR、SEM、氮氣吸附/解吸等溫線和UV-Vis測量所得粉末的晶相、形貌、表麵積和反射率。此外，通過模擬體液的pH值變化和MTT試驗研究了降解性能和細胞毒性。

    Bone disease or bone defects are serious programs of human lives. Bone graft is one of the common transplantation tissues and calcium phosphate is widely used for it because its chemical composition is similar to human bone. β-tricalcium phosphate (β-TCP) is suitable for bone graft, which is biocompatibility and bioresorbability. However, the degradation rate of β-TCP is too fast because of its good solubility in body fluid. A personalized bone graft is needed because young people have fast bone growth while older people have slow bone growth. Therefore, β-TCP and bioactive glass (BG) composites have been reported to achieve the personalized bone graft.
    In this study, β-TCP and bioactive glass (BG) granulated powder were fabricated by spray pyrolysis and spray drying. These fabrication methods are suitable for mass production and synthesis of spherical particles. At first, β-TCP and BG spherical particles were synthesized by spray pyrolysis. After that, these powders were granulated with the different weight% ratios of β-TCP/BG = 100/0, 75/25, 50/50, 25/75, 0/100 by spray drying. The resulting powders were measured by XRD, FT-IR, SEM, nitrogen adsorption/desorption isotherm, and UV-vis for investigation of crystal phase, morphology, surface area, and reflectance. In addition, the degradation property and cytotoxicity were investigated by pH change of simulated body fluid and MTT assay.

    摘要 ⅳ ABSTRACT ⅴ Acknowledgement ⅵ Contents ⅶ List of Figures ⅸ List of Tables ⅻ Chapter 1. Introduction　 1 Chapter 2. Literature review　 4 2.1 Bone graft 4 2.2 Types of bioceramic 5 2.2.1 Bioinert (Type 1) 5 2.2.1.1 Al2O3 5 2.2.1.2 ZrO2 7 2.2.2 Porous (Type 2) 11 2.2.3 Bioactive (Type 3) 15 2.2.3.1 Hydroxyapatite (HAp) 15 2.2.3.2 Bioglass 16 2.2.4 Resorbable (Type 4) 21 2.3 Synthesis method 25 2.3.1 Solid-state method 25 2.3.2 Sol-gel method 27 2.3.3 Spray pyrolysis 31 2.3.3.1 Mechanism of spray pyrolysis 32 2.3.4 Spray drying 35 2.3.4.1 Mechanism of spray pyrolysis 36 2.3.4.2 Granulation by spray drying 39 Chapter 3. Experimental procedure 40 3.1 Chemical and equipment 40 3.2 β-TCP precursor solution 42 3.3 BG precursor solution 43 3.4 Spray pyrolysis 44 3.5 Slurry preparation for spray drying 45 3.6 Spray drying 46 3.7 In vitro degradation test 47 3.7.1 pH change 47 3.8.MTT assay 49 3.9 Characterization 50 3.9.1 X-ray diffraction 50 3.9.2 Fourier transform infrared spectroscopy 52 3.9.3 Scanning electron microscope 53 3.9.4 Specific surface area measurement by nitrogen adsorption/desorption isotherm 54 3.9.5 Reflectance measurement by ultraviolet-visible spectroscopy (UV-vis) 55 Chapter 4. Results 56 4.1 XRD patterns of spray pyrolysis derived β-TCP and BG powders 56 4.2 SEM images and particle size distribution of spray pyrolysis derived β-TCP and BG powders 57 4.3 XRD patterns of spray drying derived β-TCP/BG granulation powders 60 4.4 FT-IR spectra of spray drying derived β-TCP/BG granulation powders 61 4.5 Photograph and diffuse reflectance spectra of spray drying derived β-TCP/BG granulation powders 62 4.6 SEM images and particle size distribution of spray drying derived β-TCP/BG granulation powders 64 4.7 Specific surface areas of spray drying derived β-TCP/BG granulation powders 68 4.8 pH change of spray drying derived β-TCP/BG granulation powders 69 4.9 Cell viability test of spray drying derived β-TCP/BG granulation powders 70 Chapter 5. Discussion 72 5.1 The particle shape difference between β-TCP and BG by spray pyrolysis 72 5.2 Color change of spray drying derived β-TCP/BG granulation powders after calcination 74 5.3 pH change of spray drying derived β-TCP/BG granulation powders 79 Chapter 6. Conclusion 80 Chapter 7. Future works 81 References 82

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