低能量雷射治療的發展已經超過30年，並將光治療技術應用於許多醫學領域，包括減緩疼痛與加速傷口復原，然而雷射儀器不但昂貴，同時也無法結合各種波長進行光治療，因此本實驗利用可調式發光二極體，當作光刺激研究的光源。實驗中利用不同波長及能量來探討類骨母細胞(UMR-106)經過光刺激後之粒線體活性、蛋白質合成、細胞增生與細胞貼附等行為表現，同時將檢測鹼性磷酸酶(ALPase)活性與鈣離子沉積，作為光照對骨細胞特徵表現之影響。
實驗中所使用的波長為652nm(紅光)、590nm(黃光)與415nm(藍光)，刺激能量為0.1~20 J/cm2。綜合實驗結果發現，細胞的粒線體活性、細胞增生與骨細胞特徵表現等行為表現，在藍光低能量(0.5J/cm2)刺激下，有最佳的提升效果，而在紅光與黃光照射下，最適合的刺激能量則為5 J/cm2，推測光受體分子除吸收波段不同外，對能量的需求程度也不同，因此短波長光源(藍光)利用較少的照射能量，即可刺激細胞產生特定生理反應，而紅黃光則需較高的能量。在高能量(10~20 J/cm2)刺激下，無論所使用為哪種波長對於細胞的行為表現都有抑制的情況發生。實驗中也發現，在相同能量不同功率的條件下，利用三種波長照射細胞，其生理表現皆沒有明顯的差異，因此認為在本實驗所採取的光刺激系統中，能量應為主要的決定性因素而非功率。

In this research, the adjustable LED (Light Emitting Diode) light with various energy and wavelength was used as light source to stimulate osteoblast-like cells, UMR-106. The responses of osteoblastic cells were investigated, including MTT assay, total protein synthesis, cell proliferation and cell attachment. Also, the ALPase (Alkaline phosphatase) activity and calcium deposition were also tested for the relazation of osteoblastic differentiation.
The wavelengths used in this study included 652 (red light), 590 (yellow light) and 415 nm (blue light) and the powers were 2.71, 3.89 and 4.17 mW/cm2, respectively. The energy for LED stimulus was ranged from 0.1 to 20 J/cm2. The experimental outcomes indicated that the optimal stimulation energy of blue light (0.5J/cm2) can promote the mitochondria activity, cell proliferation and expression of osteoblastic markers. However, the effective energy of red and yellow lights was higher (5J/cm2). The possible reason would be that photo-acceptors for lights with different wave length are different, so the required energy levels also diverse. On the other hand, to the light stimuli with high energy (10~20 J/cm2) would inhibit the cell activity and differentiation, no matter which wave length was used. The importance of stimulation power and energy was also investigated in this study. The results suggested that the irradiation energy is dominant, compared with the stimulation power.

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