研究生: |
張士浤 Shi-Hung Chang |
---|---|
論文名稱: |
由生質柴油餾餘物中分離米糠醇 Isolation of gamma oryzanol from residue of biodiesel |
指導教授: |
朱義旭
Yi-Hsu Ju |
口試委員: |
陳志平
Chih-Ping Chen 蔡少偉 Shao-Wei Tsai 林俊一 Chun-I Lin |
學位類別: |
碩士 Master |
系所名稱: |
工程學院 - 化學工程系 Department of Chemical Engineering |
論文出版年: | 2006 |
畢業學年度: | 94 |
語文別: | 英文 |
論文頁數: | 38 |
中文關鍵詞: | 米糠醇 、米糠油 、生質柴油 、矽膠管柱層析法 |
外文關鍵詞: | rice bran oil, biodiesel, gamma oryzanol, silica gel column chromatography |
相關次數: | 點閱:241 下載:6 |
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對於生產生質柴油,米糠油是相當便宜的原料用油。若能純化分離出米糠的高附加價值的副產物,如由萃完油的米糠中分離蛋白質,碳水化合物,或由生質柴油餾餘物中分離米糠醇,希望利用這些高附加價值的副產物進而降低生質柴油的成本。
米糠油含有1.8~3.0%米糠醇。米糠醇在食物,化妝品,製藥的工業上有重大的應用。該研究以米糠油為原料,於製造生質柴油的過程中的餾餘物分離米糠醇。在第一階段程序中,經由除膠除蠟,酸催化酯化反應,低壓蒸餾,將米糠醇的純率提高至16.18% (總回收率:83.62%),在低壓蒸餾中,95%的低沸點物質如游離脂肪酸,脂肪酸甲基酯可被移除。在餾餘物中含有高含量的甘油酯,利用酵素催化酯化反應,將甘油酯轉化為脂肪酸甲基酯。以此反應後的餾餘物為基質,利用矽膠管柱層析法,收集米糠醇。米糠醇由16.18%提高到73.75%(總回收率:66.88%)
Rice bran oil (RBO) is an inexpensive raw material for the production of biodiesel. Utilization of by-product such as defatted rice bran for the production of proteins, carbohydrates, phytochemical, and the isolation and purification of value-added nutraceuticals generated, such as -oryzanol and tocos, during biodiesel production from RBO are attractive options to lower the cost of biodiesel.
Crude RBO contains 1.8~3.0% -oryzanol. -Oryzanol has important applications in food, cosmetic and pharmaceutical industry. The object of this investigation is to isolate -oryzanol from residue obtained during the production of biodiesel from RBO. Using RBO as the starting substrate, the content of -oryzanol could be raised to 16.18 % (recovery 83.62%) by a series of steps which included degumming, acid-catalyzed esterification and vacuum distillation (3.4~3.6×10-2 torr, 192oC). In the distillation step, more than 95% of low-boiling point components, such as free fatty acid (FFA) and fatty acid methyl ester (FAME) could be distilled. After distillation, diglyceride and triglyceride in the residue were converted into FAME by enzyme (Novozyme 435) catalyzed esterification. The organic phase of the reaction product was then subjected to silica gel column chromatography. After collecting the oryzanol enriched fraction and removing the solvent, the content of -oryzanol could be raised from 16.18 % to 73.75 % with a recovery of 79.75% (total recovery 66.88%).
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