-Poly-L-lysine (-PL) is a homo-poly-amino acid characterized by the peptide bond between carboxyl and -amino groups of L-lysine. -PL is produced by several strains of Streptomyces, typically Streptomyces albulus as an extracellular product. -PL shows a wide antimicrobial activity including Gram positive, Gram negative, yeast and fungi. The minimum inhibitory concentration of -PL was measured based on broth dilution method. 62.5 mg/L of -PL was required to inhibit the growth of E. coli and B. subtilis, while 500 mg/L of -PL was required to inhibit the growth of P. pastoris.
CMC-magnetite was synthesized by mixing CMC with iron mixture solution in alkaline condition. As much as 0.0125 equivalence of carboxylic acid group was contained for each gram of CMC-magnetite. Having the diameter of 30 nm and zeta potential of -15.60 mV at pH 7, CMC-magnetite was employed to purify -PL through electrostatic interaction with the maximum capacity of 0.3788 g -PL/g CMC-magnetite. The dissociation constant of the adsorption isotherm was 0.3770 g/L and 60.77% of adsorbed -PL could be recovered from the CMC-magnetite.
-PL was employed on biomedical application. It was immobilized on microbial cellulose. 5% of NaIO4 with 2 h incubation was required to activate the microbial cellulose. As much as 5 mg of -PL was immobilized on each gram of microbial cellulose under alkaline condition. -PL-modified microbial cellulose was positive on ninhydrin assay and contained 56.06 mM amino group based on glycine calibration curve.

-Poly-L-lysine (-PL) is a homo-poly-amino acid characterized by the peptide bond between carboxyl and -amino groups of L-lysine. -PL is produced by several strains of Streptomyces, typically Streptomyces albulus as an extracellular product. -PL shows a wide antimicrobial activity including Gram positive, Gram negative, yeast and fungi. The minimum inhibitory concentration of -PL was measured based on broth dilution method. 62.5 mg/L of -PL was required to inhibit the growth of E. coli and B. subtilis, while 500 mg/L of -PL was required to inhibit the growth of P. pastoris.
CMC-magnetite was synthesized by mixing CMC with iron mixture solution in alkaline condition. As much as 0.0125 equivalence of carboxylic acid group was contained for each gram of CMC-magnetite. Having the diameter of 30 nm and zeta potential of -15.60 mV at pH 7, CMC-magnetite was employed to purify -PL through electrostatic interaction with the maximum capacity of 0.3788 g -PL/g CMC-magnetite. The dissociation constant of the adsorption isotherm was 0.3770 g/L and 60.77% of adsorbed -PL could be recovered from the CMC-magnetite.
-PL was employed on biomedical application. It was immobilized on microbial cellulose. 5% of NaIO4 with 2 h incubation was required to activate the microbial cellulose. As much as 5 mg of -PL was immobilized on each gram of microbial cellulose under alkaline condition. -PL-modified microbial cellulose was positive on ninhydrin assay and contained 56.06 mM amino group based on glycine calibration curve.

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