現今的積層製造技術(AM)已被應用於組織工程支架的製作，並去解決像是傳統方法上的孔洞控制與幾何圖形問題，大部分積層製造都是以擠出或者是燒結的方式，很少使用光固化成型，在實驗室先前研究中利用光固化PCL材料(PCL-DA)結合PEG-diacrylate(PEG-DA)並藉由動態光罩快速成型系統進行光固化，但是在材料特性上還沒完全被釐清。此外，材料堆疊層數的製作被侷限於過固化問題產生，因此本研究目的在於材料性質探討並對過固化問題進行成型性改善。
在PCL-DA+PEG-DA三種比例(6:4、7:3、8:2)的探討，利用熱分析儀器(TMA、DSC和TGA)來計算熱膨脹係數與了解熱穩定性，從60天的降解試驗中得知重量損失率與pH值，並以PCL-DA+PEG-DA薄膜的方式來進行纖維母細胞L929的體外培養，其結果可從SEM的觀察與MTT測試來證明細胞貼附與成長狀況。此外，為了改善支架製作上的成型性，提出了兩大策略來解決因材料透明而導致過固化問題─多元圖形支架幾何設計與材料染色。在多元圖形支架設計上，孔洞的多元性與連通性是有利提供細胞成長，但在過固化的影響上是被侷限的。另一方面，染色材料可以有效地阻擋光源避免支架發生過固化，支架的堆疊層數上可以從原本的八層堆疊到二十層或更多，在支架製作上已經成功的改善其成型性。

Additive Manufacturing (AM) technologies have been applied to fabricate tissue engineering scaffolds nowadays to solve issues such as pore-size control and geometry shapes in traditional methods. Most AM approaches utilize extrusion or sintering, yet few by photo-curing. In the previous research in our lab, preliminary study on photo-curable PCL material (PCL-DA) combining with PEG-diacrylate (PEG-DA) cured by Dynamic Masking Rapid Prototyping System was conducted, but material properties are not well understood. Besides, the over-curing issue of the material limited the number of layers being fabricated. Therefore, in this research, more material properties were investigated, and fabrication capability were improved.
Three ratios (6:4, 7:3, 8:2) of PCL-DA to PEG-DA were studied. Thermal analyzer equipments (TMA, DSC, and TGA) were used to determine the coefficient of thermal expansion of the materials and understand their thermal stability. Degradation tests revealed weight loss and pH value of materials within 60 days. Moreover, the L929 cells were cultures in vitro on the cured PCL-DA/PEG-DA thin-film to observe biocompatibility. The results from SEM observation and MTT tests proved cell adhesion and growth on the cured materials. Besides, in order to improve scaffold fabrication capability, two strategies were proposed to eliminate over-curing issue of transparent materials—multi-pattern geometry design and dyed materials. The multi-pattern design can provide various sizes of connecting pores for cells growth, but the effects on avoiding over-curing is limited. On the other hand, dyed materials can block the light to over-cure previously cured layers effectively and increase the stackable numbers of layers from 8 to 20 and more. The scaffold fabrication capability has successfully been improved.

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