皮膚切片是醫師常用於判斷皮膚疾病的方法，一般分為鑽取活組織(Punch biopsy)、刮削活組織(Shave biopsy)、切口活組織檢查(Incisional biopsy)，而這些皮膚切片的檢查是屬於侵入式(Invasive)的檢測方式，使得病患在檢測的過程中流血甚至留下疤痕，光學同調斷層掃描術於1991年發明至今，已在生物醫學影像上佔有一席地位，其在量測的過程中不需要特殊記號，並且能夠以非侵入式的方式得到3D立體結構生物醫療影像。
本文中使用實驗室生長的摻鉻鎂橄欖石晶體光纖以520nm綠光雷射泵浦產生放大自發輻射(Amplified spontaneous emission; ASE)作為Mirau-based全域式光學同調斷層掃瞄系統所使用的光源，共使用兩顆1-W 520nm之雷射二極體泵浦摻鉻鎂橄欖石晶體光纖，對於沿C軸方向生長的摻鉻鎂橄欖石晶體光纖可以產生1.4 mW ASE，中心波長900 nm、頻寬250 nm，可以使OCT系統上在活體組織中有1.18 μm的縱向解析度能力；沿B軸方向生長的摻鉻鎂橄欖石晶體光纖可以產生3.3 mW ASE，中心波長900 nm、頻寬200 nm，並搭配新設計架構可使OCT系統在活體組織中有1.26 μm的縱向解析度能力；再搭配40X物鏡下得到0.65μm的橫向解析度能力，而搭配20X物鏡下可得到1.31 μm的橫向解析度能力，進行活體皮膚之量測，使用20X下得到247 μm的縱向穿透能力，可以量測到角質層、表皮層與真皮層之交界等三維結構資訊。
改良目前系統設計使得有效進入待測物的光功率上升，使干涉效率由6.52%上升至25.1%，可有效抑制雜訊。 

skin biopsy is the gold standard for doctor to diagnosis skin cancers. There are several ways to do the skin biopsy such as punch biopsy, shave biopsy, incisional biopsy, incisional biopsy. These methods are all invasive that make the patient bleed and leave scar. Optical coherence tomography is one of the techniques in the biomedical imaging system. It is a noninvasive, label-free and 3D imaging method.

In this thesis, Cr:forsterite crystal fiber amplified spontaneous emission(ASE) light source was used to build a Miura-based full-field Optical coherence tomography system. using two 520-nm laser diode to pumped the different axis Cr:forsterite crystal fiber, the C-axis fiber ASE centered 900 nm with a bandwidth of 250 nm was generated. The mirau-based full-field OCT has an axial resolution of 1.18 μm in tissue
. The B-axis fiber ASE centered 900 nm with a bandwidth of 200 nm was generated. The mirau-based full-field OCT has an axial resolution of 1.26 μm in tissue. two kinds of water immersion objective lens were employed, when employed 40x mirau objective was achieved a lateral resolution of 0.65 um and 20x mirau objective was achieved a lateral resolution of 1.31 μm

Using this system, we can measure in-vivo skin and get the high-resolution 3D volume structure. Utilizing the low absorption light source, the penetration depth of in-vivo skin measurement is about 247 μm. we can us this high-resolution OCT image to quantize the thickness of stratum corneum and can distinguish the dermo-epidermal junction.
Improved of optical power of incident sample light source made the interference efficiency growth from 6.52% to 25.1% and effectively noise suppression.

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