心血管疾病是全球的主要致死原因之一，目前的醫療管理在治療心臟衰竭方面效果有限，在心肌梗塞（MI）的情況下，大量心肌細胞在短期內因缺血而死亡，對心臟功能構成重大威脅，因此，開發具有高生物相容性、自修復特性的心臟貼片以誘導心肌細胞再生顯得尤為重要。
我們的研究證明，將GOPS、DMSO及非離子界面活性劑FS3100融入聚二氧乙基噻吩:聚苯乙烯磺酸（poly(3,4-ethylenedioxythiophene) polystyrene sulfonate, PEDOT:PSS）薄膜中，賦予了心臟貼片卓越的柔韌性、可拉伸性、自修復性能及優異的導電性於生物電子醫學的應用；發現製程乾燥速度可明顯調控表面電位；此外，聚乙烯醇（PVA）水凝膠的外部施壓證實了對PEDOT:PSS複合膜的加速修復能力。
另外，我們對永生化骨髓幹細胞（IBMSC）的外泌體（exosomes）進行了尺寸排阻層析法（size exclusion chromatography, SEC）純化，並應用IBMSC來源的exosomes來證實其有助於改善缺血性損傷後的心臟組織。我們進一步探討了逐層（LbL）技術，通過exosomes與聚赖氨酸（PLL）/聚苯乙烯磺酸（PSS）的靜電吸附，以及甲基丙烯酸明膠（gelatin methacrylate, GelMA）的封裝來控制外泌體的裝載及電控釋放，GelMA水凝膠有助於減緩外泌體的釋放速率，提高外泌體的保留時間，實現持續的治療效果，為由MI引起的部分組織壞死提供了潛在的新穎修復方案，並展示了在治療心臟缺血性損傷和改善心肌纖維化方面的應用前景。

Cardiovascular diseases are among the leading causes of death globally, and current medical management for treating heart failure is often limited in efficacy. In cases of myocardial infarction (MI), large numbers of cardiomyocytes die within a short period due to ischemia, posing a significant threat to heart function. Therefore, developing highly biocompatible, self-healing cardiac patches to induce cardiomyocyte regeneration is of paramount importance.
Our research demonstrates that incorporating GOPS, DMSO, and the non-ionic surfactant FS3100 into poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) films endows the cardiac patches with exceptional flexibility, stretchability, self-healing properties, and outstanding conductivity. Additionally, polyvinyl alcohol (PVA) hydrogels have been shown to confirm the high stability and accelerated repair capabilities of the PEDOT:PSS composite films.
We purified exosomes derived from immortalized bone marrow stem cells (IBMSCs) using size exclusion chromatography (SEC). Research indicates that IBMSC-derived exosomes contribute to neovascularization and can inhibit inflammatory responses, thereby improving ischemic injury-affected cardiac tissue. We further explored the layer-by-layer (LbL) technique, employing the electrostatic adsorption of exosomes with poly-L-lysine (PLL)/polystyrene sulfonate (PSS) and encapsulation with gelatin methacrylate (GelMA) to control the release of exosomes. This approach helps to slow the release rate of exosomes, extend their retention time, and achieve sustained therapeutic effects.
These innovations provide a potentially novel repair strategy for tissue necrosis caused by MI and demonstrate promising applications in treating ischemic cardiac injuries and improving myocardial fibrosis.

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