近年來隨著石油危機和環境污染等問題的日益嚴重，人們積極尋找可以替代的能源。現今研究朝向將微生物發酵生產的醇類產物做為石油的替代能源。而這些醇類產物中又以低碳數的丁醇最具有成為替代能源的潛力。
生物丁醇通常是利用能夠會同時生產丙酮─丁醇─乙醇（Acetone─Butanol─Ethanol ,ABE）的微生物來發酵產生。但由於發酵液中丁醇濃度過低（1∼2 %），導致分離及純化需要耗費相當多能量，因此要從發酵液中分離回收丁醇具有相當大的挑戰。利用吸附劑吸附丁醇的方法可以在低耗能的狀態下有效分離丁醇，故本論文研究利用沸石咪唑骨架材料（ZIF-8）做為生物丁醇的吸附劑，探討其對丁醇水溶液及發酵液中之丁醇的吸附行為。
本研究先利用Clostridium acetobutylicum ATCC 824來發酵生產丁醇，在最佳的培養條件下進行發酵，可以得到10.8 g/L的丁醇，1.8 g/L的乙醇，3.72 g/L的丙酮和1.8 g/L的丁酸，並利用ZIF-8於此發酵液中進行吸附分離丁醇。
ZIF-8在模擬發酵液中的對丁醇的最大吸附量約為290 mg/g，但同時對也乙醇和丙酮具有微量的吸附能力。在真實發酵液中ZIF-8對丁醇的最大吸附量為208 mg/g，推測主要是丁酸鹽類會降低ZIF-8對丁醇的吸附量。
由於ZIF-8微米顆粒不易回收且在酸酸性環境下會崩解，因此本研究利用親醇性極佳的聚二甲基矽氧烷（PDMS）做為ZIF-8的載體，藉以改善此問題並製備可重複使用的丁醇吸附劑。

Bioalcohols such as ethanol and butanol are alternatives to fossil oil-based fuels. Especially biobutanol has many superior properties than that of bioethanol. Biobutanol is usually produced by acetone-butanol-ethanol (ABE) fermentation of bacterial strain Clostridium. However, its separation from the fermentation broth is still a huge challenge. Adsorption is one of the energy efficient techniques for butanol separation from dilute fermentation broths. In this study, a zeolitic imidazolate fragmentation-8 (ZIF-8) was used as an adsorbent for the separation of biobutanol from fermentation broths.
The maximum adsorption capacity of ZIF-8 for butanol was determined to be about 290 mg/g. Ethanol and acetone were observed to be adsorbed by ZIF-8 but with a small amount. Butanol of 10.8 g/L, ethanol of 1.8 g/L, acetone of 3.72 g/L and butyrate acid of 1.8 g/L were obtained in Clostridium acetobutylicum ATCC 824 fermentation broth.In fermentation broths. The maximum adsorption capacity of ZIF-8 for butanol from the fermentation broth was about 208 mg/g. The reduced butanol adsorption capacity was resulted from the presence of butyrate which will compete with butanol for adsorption on ZIF-8.
To facilitate its easy application for butanol adsorption, ZIF-8 was also mixed with polydimethylsiloxane (PDMS) to prepare a composite film on reusable butanol adsorbent. PDMS was employed because its butanol adsorption preference. Besides, PDMS not only acts as a support for ZIF-8 powder but also a barrier for uptaking butyrate from fermentation broth.

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