本研究目的為設計一種複合奈米纖維偵測大腸癌病患外周血中的循環腫瘤細胞(CTCs)，然而循環腫瘤細胞的偵測往往會受到大量白血球的影響。複合奈米纖維分別由尼龍6 (Nylon-6)、聚乙烯乙二醇(Poly(ethylene oxide)；PEO)所構成，其中尼龍6 (Nylon-6)是做為檢測的基底材料，用於防止奈米纖維水解於水中。聚乙烯乙二醇(Poly(ethylene oxide)作為生物抗沾黏材料，且表面改質後仍有生物抗沾黏功能可避免白血球沾附，PEO經過(3-aminopropyl)triethoxysilane (APTES)修飾後帶有NH2官能基可固定Streptavidin和anti EpCAM來偵測CTCs。將此兩種材料以甲酸為溶劑用不同的比例進行混合，利用靜電紡絲製造出纖維薄膜，達到不易水解、生物抗沾黏、且富含anti EpCAM抗體的目的，Nylon-6/PEO最佳的混合比例為30/70。
首先測試奈米纖維抗白血球沾附的能力，發現PEO整體平均具有90%的抗白血球沾黏能力。再進一步拿癌細胞株(HCT-116、DLD-1、SAS和Hela)進行檢測，證明本材料可在環境中含有10到10萬顆的情況下來偵測CTCs，其結果為高達70%的抓取效率。
最終進行21次臨床實驗，得知只要是大腸癌病人便一定可抓到循環腫瘤細胞，本複合奈米纖維有著低成本且檢測快速的優勢，未來具有高度發展潛力成為新參考指標，並希望能和現行大腸癌參考指標；CEA腫瘤標記成為雙指標。

In this study, we designed an electrospinning composite nanofiber to capture colon circulating tumor cells (CTCs) from peripheral blood of colon cancer patients. Detection of CTCs is always interfered by lots of leukocyte contaning in human blood. We introduced nylon-6 and poly(ethylene oxide)(PEO) to electrospin as nanofiber membranes. Nylon-6 is a substrate which can avoid the nanofibers to dissolve in aqueous solution. PEO and derivatives are anti-fouling material to prevent leukocyte fouling. For grafting amine groups, PEO is modified with (3-aminopropyl)triethoxysilane (APTES). Amine groups are media to fix antibody biotin(EpCAM biotin) to caught the CTCs. The result suggest that the radio of Nylon6/PEO is 30/70.
After testing, we find nanofibers possessing the ability to antifoul of leukocyte. The efficiency is 90%. Colon cancer cell line (HCT-116,DLD-1,SAS and Hela), 10 to 100000 colon cancer cells, are mixed in 0.3ml medium to perform the capture of CTCs. The results show that detection of efficiency increase linearly upon 70%.
Finally, we started clinical tests for 21 times. If the blood come from colon cancer patients, we will find CTCs. There are some advantage in our nanofibers. For example, we will know the result in the short time,and the cost of nanofibers are very low. We trust our nanofibers possessing high potential being a new reference for colon cancer patients like CEA.

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