在本篇論文中，吾人以帶有不同應力影響之磊晶石墨烯奈米牆(epitaxial grapheme nanowalls, EGNWs)作為電極，並將葡萄糖氧化酶酵素(glucose oxidase)固定於其表面後應用於葡萄糖生物感測；吾人利用石墨烯奈米牆之三維高表面積結構、高活性特性之特點增加了酵素與電極間之電荷轉移速率(5.43 ± 0.31s-1)以及反應活性，並於葡萄糖感測中除了增加對葡萄糖之敏感度(41.09 ± 0.23μA/mM/cm2)以及降低了濃度感測極限至~2pM之外，更增加了酵素對於葡萄糖之親和性(0.291 ± 0.018 mM)；同時經由實驗發現應力變化可影響酵素與石墨烯之間的電子傳導特性，進而改變葡萄糖感測之行為，其中又以具有張應力(tensile strain)之石墨烯奈米牆電極對於葡萄糖感測效果為最佳。

In this work, few-layer epitaxial graphene nanowall (EGNW) arrays, a 3D wall-like edge-oriented hetero-architecture consisting of few-layer graphene grown on silicon carbide, by microwave plasma-enhanced chemical vapor deposition (MPECVD), with different strain effect by tuning different H2/CH4 ratio during process, were demonstrated by Raman spectroscopy. This material was employed for glucose sensing due to its larger surface area, controllable layer number and highly graphitic nature of epitaxially grown graphene, and enhanced edges of nanowall-architecture. The work showed that the enzyme-modified EGNW electrode could provide a remarkably improved contact (ks= 5.43 ± 0.31s-1) with the glucose oxidase (GOD). In the glucose analysis, the GOD/EGNWs has a sensitivity of 41.09 ± 0.23μA/mM/cm2, a largest detection range from 2 pM to 5mM, and a glucose affinity of 0.291 ± 0.018 mM. Furthermore, the strain effect of EGNWs for glucose sensing could be observed by not only in charge transfer between GOD and EGNWs but also in glucose sensing. The tensile strain EGNWs shows much better electronic transport, sensitivity, and glucose affinity than the other materials.

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