表面增強拉曼光譜檢測(SERS)在化學成分分析中扮演著重要的角色。相較於普通的塊材拉曼，SERS基板對於拉曼訊號的增幅使得它將擁有更大的優勢。這類基板通常都是奈米尺度的金屬微結構覆蓋在矽或者玻璃基板上。
當激發光入射至這類基板的表面時，表面電漿共振現象就會對拉曼訊號強度進行增幅。然而，這類基板的價格往往是進行普及化量測上的一大障礙。因此，選擇一種價格低廉的SERS基板是透過奈米壓印技術及聚合物材質製成的，將在整個拉曼量測的實際應用中具備極大的潛力。
然而，由於此種基板具有較差的熱傳導效率，因此容易導致量測時的入射光點處堆積了大量的熱而破壞待測物(或SERS基板)。在本研究中，我們開發了一種基於反射式光學元件的新式拉曼探頭，此探頭能夠大幅降低入射光點的能量密度，並且將與一般傳統的顯微拉曼系統進行一系列的比較來證明新式探頭的優勢。本研究也將深度探討能量密度對於SERS基板在訊號強度所帶來的負面影響以及基板破壞的現象。

Surface-Enhanced Raman Spectroscopy (SERS) is a powerful measurement method in the chemical analysis field. It is much superior to bulk Raman owing to the enhancement of signal sensitivity from the SERS substrate. The substrate is usually designed with nanoscale metal structures coated on silicon or glass substrates.
When excitation light incident the surface of the substrate, surface plasmon resonance (SPR) occurs, and meanwhile, the signal from Raman scattering is enhanced. Nevertheless, the costs of those delicate SERS substrates are overpriced, which results in difficulty of universal measurement. Accordingly, opting for a substrate made of polymer material based on the "Nanoimprint" technique shows great potential in future applications.
However, the polymer’s thermal property may cause heat to concentrate on the incident spot and damage the analyte (or SERS substrates) due to its low heat conductivity. In this article, we proposed a novel design of the Raman optical probe based on reflective optics to reduce the power density. The proposed optical probe was directly compared with a regular micro Raman system and demonstrated its unique stability.
Moreover, the efficiency of our home-build Raman probe is equivalent to the commercial product. Finally, a deep analysis relating to the heat impact on the SERS signal and surface damage will be discussed.

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