聚乳酸/魚鱗粉骨生醫複合材料之開發研究｜國立臺灣科技大學博碩士論文系統

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研究生：	黃芷翎 Jhih-Ling Huang
論文名稱：	聚乳酸/魚鱗粉骨生醫複合材料之開發研究 Development of Poly (lactic acid)/Scales Powder Composites Apply to Bone Biomedical Materials
指導教授：	楊銘乾 Ming-Chien Yang
口試委員:	吳昌謀 Chang-Mou Wu 鄒智揮 Chi-Hui Tsou
學位類別：	碩士 Master
系所名稱：	工程學院 - 材料科學與工程系 Department of Materials Science and Engineering
論文出版年：	2016
畢業學年度：	104
語文別：	中文
論文頁數：	79
中文關鍵詞：	聚乳酸、魚鱗粉、氫氧基磷灰石
外文關鍵詞：	Polylactic acid, Scales Powder, Hydroapatite
相關次數：	點閱：244 下載：6
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本研究以融熔混煉方式製備聚乳酸/魚鱗粉 (PLA/SP)複合材料，其中 SP 重量百分比含量為 0 至 50 wt%。以熱重損失分析儀 TGA 分析熱裂解溫度、熱殘餘量和 SP 中明膠和氫氧基磷灰石的比例；此外DSC 分析以速率 2 ℃/min 探討熔融結晶及熔融行為；並以 Avrami equation 描述等溫結晶動力學，分析結晶速率和平衡熔點；以 XRD、FTIR 分析 SP 特徵峰和官能基證明其成分為明膠和氫氧基磷灰石；以拉伸試驗測量機械性質的變化；將樣品浸泡在 PBS 中 4 週、8 週、12週做穩定性分析，並測量其重量損失計算降解率；用 SEM 觀察複合材料的斷面和穩定性分析後的複合材料表面、斷面；由生物相容性測試可看出添加 SP 與否不會影響 PLA 的生物相容性，並由細胞貼附測試觀察細胞貼附生長情形，可望此複合材料能應用於骨骼修復之射出級醫療產品。

In this study, polylactic acid (PLA)/scales powder (SP) composites were prepared by melt blending. The SP content of composites are from 0 to 50 wt%. The thermal stability and the residual amount of the PLA and the PLA/SP composites were studied by using a Thermo gravimetric Analyzer. Moreover, differential scanning calorimeter (DSC) analysis at a rate of 2 ℃/min to discussion melt crystallization behavior, melting behavior and isothermal crystallization kinetics. Increased concentration of SP reduced crystal formation of PLA as seen by the peak intensity of X-ray diffraction (XRD). The functional groups of SP and PLA were analyzed by Fourier transform infrared spectroscopy (FTIR). The tensile test is to find the measurement of various mechanical properties. The samples were soaked in phosphate buffer solution (PBS) for 4 weeks, 8 weeks and 12 weeks to study stability analysis, and measuring degradation rate. Microstructure characterization of cross-sections of the PLA and the PLA/SP composites were observed by scanning electron microscopy (SEM). The in vitro cytotoxicity test show that the SP adding would not affect the PLA biocompatibility by L929. We expected this composites can be used in bone repair of injection grade medical products.

摘要	Ⅰ
ABSTRACT	Ⅱ
致謝	Ⅲ
總目錄	Ⅳ
圖目錄	Ⅶ
表目錄	Ⅸ
第一章 緒論	1
    1-1 前言	1
    1-2 研究目的	2
第二章 研究背景	3
    2-1熔融混煉原理與等溫結晶動力學	3
        2-1-1 熔融混煉原理	3
        2-1-2 等溫結晶動力學	5
            2-1-2-1 晶體成長	5
            2-1-2-2 Avrami方程式	8
            2-1-2-3 平衡熔點	10
    2-2 骨組織工程與生醫材料發展	11
        2-2-1 組織工程	11
        2-2-2 骨組織與骨再生	13
        2-2-3 骨移植及骨修補	16
        2-2-4 骨生醫材料的發展	18
    2-3 實驗材料介紹	21
        2-3-1 聚乳酸	21
        2-3-2 台灣鯛魚鱗粉	23
        2-3-3 氫氧基磷灰石	24
        2-3-4 明膠	26
第三章 實驗	27
    3-1 實驗材料與藥品	27
    3-2實驗儀器	28
    3-3實驗流程	29
    3-4材料配置	30
        3-4-1熔融混煉步驟	30
        3-4-2自動熱壓成型步驟	30
    3-5材料物性測試	31
        3-5-1熱重損失分析	31
        3-5-2差示掃描量熱分析	32
        3-5-3 X光繞射分析	33
        3-5-4傅立葉紅外線光譜分析	33
        3-5-5掃描式電子顯微鏡分析與能量分散譜分析	34
        3-5-6拉伸強力分析	34
        3-5-7穩定度測試分析	35
        3-5-8接觸角分析	36
    3-6細胞相容性	37
        3-6-1細胞株來源與培養條件	37
        3-6-2細胞株繼代與冷凍保存	37
        3-6-3 MTT細胞活性測試	38
        3-6-4體外細胞毒性	39
        3-6-5細胞表面型態分析	40
第四章 結果與討論	41
    4-1熱重損失分析	41
    4-2熱學性質分析	43
    4-3等溫結晶動力學	46
        4-3-1結晶速率分析	46
        4-3-2 Avrami分析	49
        4-3-3 平衡熔點	53
    4-4 X光繞射分析	55
    4-5傅立葉紅外光譜分析	57
    4-6拉伸強力分析	59
    4-7粒徑&表面結構分析	60
    4-8能量分散譜分析	62
    4-9穩定度測試	65
    4-10接觸角分析	69
    4-11體外細胞毒性測試	70
    4-12細胞貼附測試	71
第五章 結論	72
第六章 參考文獻	76

                                

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