金奈米粒子( Gold nanoparticles, AuNPs )具有化學惰性和生物相容性，在生物傳感器、藥物輸送等方面具有重要的醫學應用價值。當具10 -100奈米尺寸的金奈米粒子暴露在光輻射下，金奈米粒子內的電子會受電場的驅動而產生震盪，並在金奈米粒子表面產生表面電漿，其進而導致它們吸收和散射光(表面等離子共振)。本研究採用綠色化學方法合成了confeito-like金奈米粒子。利用紫外-可見光譜和透射電子顯微鏡對confeito-like的金奈米粒子進行分析。Confeito-like的金奈米粒子平均粒徑約40 nm且在500至700 nm處具有表面等離子體激元帶(surface plasmon band)。紫質(Porphyrins,TCPP)是一種典型的光敏藥物，在415 nm處具有Soret吸收帶且在510、550、580和640 nm附近具有4個較小的Q帶(Q bands)，其可用於修飾金奈米粒子。本研究旨在合成TCPP共軛的confeito-like金奈米粒子，以應用於光動力療法(photodynamic therapy)。利用半胱氨酸(L-cysteine)和碳點(carbon dots)保護的氧化鐵(Fe3O4@C)共價鍵結，製備了TCPP共軛的confeito-like的金奈米粒子 (Au@Cys@TCPP和Au@FeC@TCPP)。透過產生單重態氧(singlet oxygen)來評估TCPP與confeito-like金奈米粒子之有效性地結合。結果表明， TCPP共軛的confeito-like的金奈米粒子及confeito-like的金奈米粒子於光動力療法應用中具有發展潛力。

Gold nanoparticles (AuNPs) possess the characteristic of both inertness and biocompatibility which have a great medical application in the biosensor and drug delivery. When AuNPs with 10-100 nm in size are exposed to optical radiation, the electrons within the AuNPs resonate, causing them to absorb and scatter the light (called the surface plasmon resonance). In biomedicine, this unique optical property can be exploited for application such as photodynamic therapy. In this study, confeito-like gold nanoparticles were synthesized by green chemistry method. UV-vis absorption spectroscopy and transmission electron microscopy were used to characterization of confeito-like AuNPs.
This study aims the synthesis of porphyrin-conjugated confeito-like AuNPs (Au@Cys@TCPP and Au@FeC@TCPP) for photodynamic therapy (PDT) application. They were prepared by covalent binding, with using L-cysteine and carbon dots-protected iron oxide (FeC or Fe3O¬4@C) as adapted linkages. For L-cysteine, the thiol group of L-cysteine was bound to confeito-like AuNPs and also formed amide bonding between porphyrin TCPP and L-cysteine to obtained Au@Cys@TCPP which was confirmed by FT-IR spectroscopy. On the other hand, co-precipitation procedure was the method to obtain magnetic Fe3O4 particles and the hydrothermal procedure was used for the synthesis of Fe3O4@C. Fe¬3O4@C has carboxylic and amine groups which can be formed the amide bonding between confeito-like AuNPs and porphyrin TCPP. Both of porphyrin-conjugated confeito-like AuNPs were characterized by using FT-IR spectroscopy, UV-vis spectroscopy and transmission electron microscopy. The effectiveness of the porphyrin-conjugated confeito-like gold nanoparticles was evaluated by the singlet oxygen generation. These results indicate a highly potential of not only porphyrin-conjugated confeito-like AuNPs but also confeito-like AuNPs for PDT.

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