傷口換藥為促進傷口癒合的必要措施，但是當敷料沾黏於傷口上時移除敷料的動作會對傷口產生剪力導致組織受損，若能提供增加護理人員辨識敷料狀態的方案，將會有助於減少病人換藥的次數。本研究成功利用鞣花酸、聚乙烯醇、絲素蛋白與絲膠蛋白開發一項具有指示功能的組合敷料，其中包含不織布指示層與泡綿接觸層，不織布指示層由絲素蛋白與聚乙烯醇利用靜電紡絲技術堆疊形成，並將鞣花酸附加於不織布表面，泡綿接觸層由絲膠蛋白與聚乙烯醇混合製備形成，可以做運用於滲出液的吸收與控制。利用紫外光吸收光譜與螢光放光光譜評估組合敷料吸收模擬體液前的螢光反應和吸收模擬體液後的螢光淬滅，在乾燥狀態下綠螢光的強度比例會提升，整體螢光放光光譜會發生紅移形成螢光反應，吸收模擬體液後綠螢光的強度比例會下降，波長與沒有鞣花酸的部分相似，形成螢光淬滅。在紫外光吸收光譜、FTIR與XPS結果中推論分子之間的作用力，並分析共價結構在過程中引發螢光反應與螢光淬滅的化學原理。利用四甲基偶氮唑鹽微量酶反應比色法評估鞣花酸、聚乙烯醇、絲素蛋白與絲膠蛋白的生物相容性，確定絲膠蛋白同時具有生物相容性與輔助細胞增生的能力。動物實驗評估市售棉墊、不織布與組合敷料的指示功能與傷口癒合效果，實驗結果中組合敷料組傷口面積的縮減率明顯高於其他組別，證明組合敷料具有促進傷口的復原的能力。

In clinical, wound dressing is a necessary medical material to protect and promote wound healing, but postoperative adhesions of dressing cause serious complications in the process of wound healing. It has necessity to provide an dressing material which can reduce requirement of dressing exchange. In this study, we developed an indicative combination dressing which was composed of indicative non-woven and foam dressing. Indicative non-woven mat was fabricated by silk fibroin protein (SFP) and polyvinyl alcohol (PVA) via the single‐spinneret electrospinning technique. The indicative function of non-woven mat was provided by ellagic acid (EA) which is a kind of polyphenols. Foam dressing was an absorbent layer composed of sericin that could control the wettability of wound microenvironment. The indicative function of dressing was attributed to the fluorescence emission which was proved by ascend of UV absorption spectrum and fluorescence emission spectrum. Result of indicative function could be elucidiated by UV absorption spectroscopy, Fourier Transform Infrared Spectrometer and X-ray photoelectron spectroscopy. Our preliminary results indicated that the combination dressing shows potential as a highly hydrophilic, excellent absorptive and low cost wound management material.

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