巴拉刈 (paraquat) 是一種劇毒的除草劑，巴拉刈中毒通常是致命的，並且是一項公共的議題。透過即時分析尿液/血漿中的巴拉刈濃度，快速確認並即時開始治療，可以改善患者的預後。
然而，現今檢測巴拉刈濃度的量測非常費時且難以實行，所需的設備昂貴且笨重。為了應對這些實際的挑戰，希望可以透過可攜式可見光光譜儀系統來解決。本論文將 Spectrochip 與商用機台 NanoDrop 進行比較，使用含有巴拉刈之臨床檢體，進行量測。結果顯示自製可攜式光譜儀系統 (Spectrochip) 其巴拉刈濃度量測檢量線R^2值為 0.9888，檢測極限為 2.07 ppm，定量極限為 6.89 ppm。並透過 Bland-Altman分析來比對結果，實驗結果與商用機台 NanoDrop 無顯著差異，兩台機台結果皆落在95% 之一致性界限內。結果證實其自製光譜儀系統是準確的、有效率且便於攜帶且成本相對下降，對於未來應用於即時檢測巴拉刈是可的。

Paraquat is a highly toxic herbicide. Paraquat poisoning is usually fatal and a publicissue. Through real-time analysis of the concentration of paraquat in the urine, rapid confirmation and immediate start of treatment can improve the patient’s prognosis.
However, the current measurement of the concentration of paraquat is time-consuming
and difficult to implement, and the equipment is expensive and bulky. In order to cope with these practical challenges, the self-made portable spectrometer system can be used to solvethem. This paper compares the self-made visible spectrometer system(Spectrochip) with the commercial machine NanoDrop. The clinical sample containing paraquat is used for measurement.The results show that Spectrochip has a calibration curve R^2 of 0.9888, a limit of detection 2.07 ppm, and a limit of quantification 6.89 ppm.The results are analyzed by BlandAltman, and the experimental results are found to be comparable to those of the commercial machine NanoDrop.There is no significant difference between NanoDrop and Spectrochip,and the results of the two machines are both within the 95% confidence interval. The results confirmed that its self-made spectrometer system is accurate, efficient, portable, and relatively low in cost, and it is feasible for future applications in real-time detection of paraquat.

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