本論文主要由兩個部分組成，第一部分論文之研究目標為，分析臨床大尺寸直徑隱形眼鏡蛋白質貼附對於病患的影響，分析組別包含三個群組，分別為：正常角膜、圓錐角膜、術後角膜。利用X-射線光電子能譜儀 (XPS)，分析配戴後之隱形眼鏡表面化學元素組成與含量，並以掃描式電子顯微鏡 (SEM) 以及原子力顯微鏡 (AFM)，觀測硬式隱形眼鏡表面形態以及粗糙度。第二部分之研究為，製備具抗蛋白質沾黏之多層高分子薄膜 (multilayer polymers thin films)，透過真空電漿聚合 (vacuum plasma polymerization) 並控制不同沉積時間與每層膜之厚度，以丙烯酸 (acrylic acid, AAc) 以及丙烯胺 (allylamine, AAm) 作為電漿聚合之前驅物，沉積於基材表面，形成ppAAc (plasma polymerized acrylic acid) 以及ppAAm (plasma polymerized allylamine) 高分子薄膜，以橢圓偏光儀 (ES)、水接觸角量測儀 (WCA)、全反射式傅立葉紅外線光譜儀 (ATR-FTIR) 等儀器，分析高分子薄膜之化學結構、表面親疏水性質以及薄膜厚度。
在論文第一部分，從XPS的分析結果發現，隱形眼鏡經三組受試者配戴六個月後，鏡片本身Si2p的波峰皆消失，此一結果顯示鏡片表面已被沉澱物所覆蓋，而這些沉澱物的內容物可能包含：蛋白質、脂質以及其他有機無機物等。另外，經角膜手術後族群配戴隱形眼鏡六個月後，可觀察到明顯的N1s之訊號，且N1s含量相較於其他族群之分析結果較高，因此，可推測角膜手術後之族群會分泌較多的蛋白質在隱形眼鏡上，使得N1s含量較高。
在論文第二部分，經由真空電漿聚合AAc以及AAm高分子薄膜，並應用於抗蛋白質沾黏。結果顯示在BSA貼附的組別中，當沉積pp(AAm10/AAc10)8時，有最低的蛋白質貼附量約1.5 μg/cm2，然而在lysozyme的組別中，當沉積pp(AAm10/AAc10)4時，有最低的蛋白質貼附量約1.2 μg/cm2。從結果中可得知沉積pp(AAm/AAc) 兩性高分子薄膜，可有效抗蛋白質沾黏之效果。

Biomaterials such as contact lenses and biosensors are commonly suffered from biofouling problems, which lead to the product contamination, equipment dysfunction, and corrosion. This thesis was divided into two parts, at the first part, the scleral contact lenses were used as analytical material. The chemical composition, surface roughness, and surface morphology of contact lenses were characterized by XPS, AFM, and SEM, respectively. For the second part, zwitterionic polymers were synthesized by vacuum plasma polymerization. The feasibility to deposit positively charged allylamine (AAm) and negatively charged acrylic acid (AAc) monomers on different substrates by plasma-assisted polymerization technique was demonstrated for bio-related applications.
For the first part of the thesis, the result from the XPS shows that the characteristic peaks of Si2p, which belong to contact lenses, disappeared after wearing for six months. And the characteristic peaks of N1s, which belong to protein, can be observed after wearing. The reason is due to the contact lenses surface was covered by the contaminants of proteins, lipids, organic and inorganic substances.
For the second part of the thesis, the feasibility to deposit positively charged AAm and negatively charged AAc monomers on different substrates by plasma-assisted polymerization technique was demonstrated for bio-related applications. The results of protein adsorption indicated that the sample pp(AAm10/AAc10)8 shows the lowest amount of protein attached onto the surface, which is 1.5 μg/cm2 by using BSA (bovine serum albumin). On the other hand, the pp(AAm10/AAc10)4 shows the lowest amount of protein attached onto the surface, which is 1.2 μg/cm2 by using lysozyme. The results show that zwitterionic polymer thin film pp(AAm/AAc) possessing good antifouling property.

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