微尺度流體混合在現今的生物晶片領域中扮演一個極為重要的角色，流體混合的效率很大程度影響了生物晶片的性能；為了普及微流體混合晶片與改善混合效率，本論文設計了一種嶄新的、低成本且高效率的微流道混合晶片。此種晶片是利用磁性人工纖毛與外加的磁場線圈系統，在流場內產生擾動造成流體混合。晶片的製造方法為機械加工式的翻模轉印製程，與以往微流體晶片常用的蝕刻製程相較起來，具有低成本、低汙染、製造快速等優勢，對於晶片的量產應用有莫大助益。此新型晶片共有兩種形式，分別為單排式以及陣列式；單排式晶片利用磁性纖毛的擾動直接延展流體介面，強制將兩種流體攪拌混合；陣列式晶片利用陣列結構串聯纖毛高速選轉產生的流場擾動，在流場中形成多個渦漩結構，將兩種流體牽引進入漩渦當中造成混合。在量測分析方面，本論文利用微尺度粒子影像測速儀 (Micro Partical Image Velocimetry, μPIV) 來進行流體速度場的量測，以瞭解混合晶片內部流場的運動行為，進一步確認提高混合效率的原因；混合效率利用螢光染色劑與雷射，以亮度差分析流體濃度從而計算混合效率。實驗結果指出，此種以磁力驅動的纖毛結構不論以單排式或陣列式運作，皆能有效地提升混合效率。本論文在此種晶片設計的方法上奠定了一個基礎，未來利用相同原理設計之新型晶片將可更快速地投入微流體晶片的應用之中。

In recent years, Biochip was growing development. The application of micromixing becomes more and more important. This thesis invented a new micromixing strategy by using artificial cilia and magnetic coil system. The artificial cilia microfluidics chip has the advantages of high mixing performance, low cost, and rapid manufacturing. This thesis designed two distributing types of artificial cilia in microchannel to enhance the mixing performance of the microfluidics. First type is one column distribution along flowing direction, which could extend the flow interface by artificial cilia stirring to enhance molecular diffusion. Second type was array distribution, which could generate some vortices to mix two working fluids. By measuring the mixing performance of each type and analyzing the principles of mixing. There were some results for this thesis: The one column distribution micromixer enhanced the mixing performance from 0.64 to 0.88, and the array distribution micromixer enhanced mixing performance from 0.42 to 0.87. These results demonstrate the new micromixing strategy by using magnetically actuated artificial cilia is quiet effective in micromixing.

[1] Gobby, D., Angeli, P., and Gavriilidis, A., 2001, "Mixing characteristics of T-type microfluidic mixers," Journal of Micromechanics and Microengineering, Vol. 11, pp. 126.
[2] Schonfeld, F., Hessel, V., and Hofmann, C., 2004, "An optimised split-and-recombine micro-mixer with uniform 'chaotic' mixing," Lab on a Chip, Vol. 4, pp. 65-69.
[3] Lob, P., Drese, K. S., Hessel, V., Hardt, S., Hofmann, C., Lowe, H., Schenk, R., Sch?Nfeld, F., and Werner, B., 2004, "Steering of liquid mixing speed in interdigital micro mixers: from very fast to deliberately slow mixing," Chemical Engineering & Technology, Vol. 27, pp. 340-345.
[4] Bessoth, F., 1999, "Microstructure for efficient continuous flow mixing," Analytical Communications, Vol. 36, pp. 213-215.
[5] Mengeaud, V., Josserand, J., and Girault, H. H., 2002, "Mixing processes in a zigzag microchannel: finite element simulations and optical study," Analytical Chemistry, Vol. 74, pp. 4279-4286.
[6] Song, H., Yin, X.-Z., and Bennett, D. J., 2008, "Optimization analysis of the staggered herringbone micromixer based on the slip-driven method," Chemical Engineering Research and Design, Vol. 86, pp. 883-891.
[7] Goullet, A., Glasgow, I., and Aubry, N., 2005, "Dynamics of microfluidic mixing using time pulsing," Discrete and Continuous Dynamical Systems, Supplement, Vol. 2005, pp. 327-336.
[8] Liu, R. H., Yang, J., Pindera, M. Z., Athavale, M., and Grodzinski, P., 2002, "Bubble-induced acoustic micromixing," Lab on a Chip, Vol. 2, pp. 151-157.
[9] Oddy, M., Santiago, J., and Mikkelsen, J., 2001, "Electrokinetic instability micromixing," Analytical Chemistry, Vol. 73, pp. 5822-5832.
[10] Deval, J., Tabeling, P., and Ho, C.-M., "A dielectrophoretic chaotic mixer," in Micro Electro Mechanical Systems, 2002. The Fifteenth IEEE International Conference on, 2002, pp. 36-39.
[11] Paik, P., Pamula, V. K., Pollack, M. G., and Fair, R. B., 2003, "Electrowetting-based droplet mixers for microfluidic systems," Lab on a Chip, Vol. 3, pp. 28-33.
[12] Ryu, K. S., Shaikh, K., Goluch, E., Fan, Z., and Liu, C., 2004, "Micro magnetic stir-bar mixer integrated with parylene microfluidic channels," Lab on a Chip, Vol. 4, pp. 608-613.
[13] Chen, C.-Y., Yao, C.-Y., Lin, C.-Y., and Wang, C.-W, 2014, "Real-time remote control of artificial cilia actuation using fingertip drawing for efficient micromixing," JALA-Journal of Laboratory Automation (in press).
[14] Sfakiotakis, M., Lane, D. M., and Davies, J. B. C., 1999, "Review of fish swimming modes for aquatic locomotion," Oceanic Engineering, IEEE Journal of, Vol. 24, pp. 237-252.
[15] Chen, C.-Y., Chen, C.-Y., Lin, C.-Y., and Hu, Y.-T., 2013, "Magnetically actuated artificial cilia for optimum mixing performance in microfluidics," Lab on a Chip, Vol. 13, pp. 2834-2839.
[16] Fang, Y., Ye, Y., Shen, R., Zhu, P., Guo, R., Hu, Y., and Wu, L., 2012, "Mixing enhancement by simple periodic geometric features in microchannels," Chemical Engineering Journal, Vol. 187, pp. 306-310.
[17] Chen, C.-Y., Lin, C.-Y., and Hu, Y.-T., 2014, "Inducing 3D vortical flow patterns with 2D asymmetric actuation of artificial cilia for high-performance active micromixing," Experiments in Fluids (in press).
[18] Lima, R., Wada, S., Tsubota, K.-i., and Yamaguchi, T., 2006, "Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel," Measurement Science and Technology, Vol. 17, pp. 797.