本研究重點是改良既有的拋棄式體外檢測晶片(In-Vitro Diagnosis Chip)之結合製程，並希望將此製程延伸至量產製程上，用於生醫產業之檢測晶片發展。研究初期的製程先利用微铣削機器對PMMA(聚甲基丙烯酸甲酯)基板進行微流道及微結構的製作，由切削的方式快速取得本研究製程測試的晶片。未來晶片的製作步驟可利用射出成形製程替代，增加晶片的製作效率來達到量產的最終目的。晶片的製作完成後，晶片黏接的部分是利用旋轉塗佈的方式，塗佈出均勻的UV膠(紫外光固化膠)薄膜層於另一塊PMMA基板上，再將兩塊基板對齊貼合後，施予一均勻的壓力，確保兩塊晶片間的貼合，最後再利用紫外光曝光機對UV膠照射定量之UV光後，使UV膠層快速固化，即完成拋棄式體外檢測晶片之製造。製程中利用多段旋轉塗佈參數的調整與間隙微小凸起結構的設計，來改善現有文獻中紫外光固化膠流入微流道的狀況，達到量產拋棄式體外檢測晶片的結合技術。

The major focus of this research is to improve the current bonding technique of disposable in-vitro diagnosis (IVD) biochips and to extend this technique into a mass production manufacturing process. A micromilling machine was used to fabricate microchannels and designed microstructures on the PMMA substrate, and a second piece of PMMA substrate was bonded to the first piece by applying UV glue bonding. To prevent the glue flowing into the microchannel during the process, two approaches were used, including designing microstructures to maintain a gap between the two pieces of PMMA substrates and spin-coating the UV glue as flat as possible. To understand the bonding strength, multiple experiments were carried out, and the results showed that those microfluidic chips could survive under the pressure of 10 bar.

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