In this thesis, a thorough exploration of dielectrophoresis phenomena is conducted, starting with the design and analysis of a 2D microchannel for contactless dielectrophoresis (cDEP) processes to observe various parameters affecting cell deflection. Subsequently, a 3D planar electrode design is developed, and critical factors influencing the cell deflection rate are calculated with a novel approach of pitch calculation. The study further delves into significant parameters such as electrode pitch, angles, voltages, cell types (cell diameter, conductivity, permeability, etc.), and frequencies. Additionally, the behavior of positive (pDEP) and negative (nDEP) dielectrophoresis in planar electrodes concerning cell deflection and its direction has been explored by analyzing a simplified model with only two electrodes. The obtained results yield valuable insights into comprehending cell deflection while traversing planar electrodes. The results reveal that the DEP force exerts influence on the planar electrodes in both directions. It acts as an attractive force for pDEP cases on both sides, making the latter electrode more influenced by DEP force compared to the preceding electrode.
Consequently, the deflection caused by the later electrode is notably higher than that of the former one. Conversely, in nDEP cases, it manifests as a repulsive force on both sides. The results shows that the first electrode is the most affected by the DEP force compared to the other electrodes. The initial electrode pushes the cell upwards, causing the cells to experience less DEP force on the subsequent electrodes. Hence, the deflection caused by the first electrode is maximum in nDEP mechanism. This revelation of force interaction contributes significantly to the field of dielectrophoresis research, shedding light on aspects previously unexplored by researchers.

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