本研究目的為設計一種複合奈米纖維偵測大腸癌病患外周血中的循環腫瘤細胞(CTCs)，而CTCs偵測容易伴隨著許多白細胞的干擾。複合奈米纖維分別是由尼龍6 (Nylon-6)、聚丙烯酸 (Poly acrylic acid; PAA)與聚甲基丙烯酸磺基甜菜鹼 (Poly sulfobetaine methacrylate; PSBMA)所構成，其中Nylon-6是做為檢測基底材料，其用於防止奈米纖維水解於水中。PSBMA為抗生物沾黏材料，亦可以避免白血球沾附。PAA上的COOH官能基可以固定streptavidin與anti EpCAM biotin來進行CTCs的偵測。將此三種材料利用甲酸為溶劑依照不同比例混合，利用靜電紡絲製造出奈米纖維薄膜，以達到不水解、抗沾黏與富含anti EpCAM抗體的目的，Nylon-6/PSBMA/PAA的最佳混合比例為50/25/25。
　　首先測試奈米纖維之抗生物沾黏能力，其發現具有高達84.8%抗血纖維蛋白原、82.4%抗內皮細胞與96.3%抗白細胞的抗沾黏能力。而進一步將三種大腸癌細胞株(DLD-1、HCT-116與HT-29)進行檢測，證實奈米纖維可從環境中僅有10至100顆的情況下來偵測CTCs，其結果為線性趨勢與高達65%的偵測效率。而在奈米纖維偵測外周血中的CTCs中，更發現了PSBMA的存在可以提高18.4%偵測能力。
　　最後進行臨床實驗，偵測三位大腸癌患者(分別為第一期的女性、第二期的女性與第三期的女性)，其每毫升血液中的CTCs數目個別為3.2、0與6.6。目前在血液中抓取CTC所面臨的阻礙，包含步驟繁雜與高成本，相對於複合奈米纖維的設計對於臨床上稀少的循環腫瘤細胞偵測是具備高度發展潛力的。

　　In this study, we designed an electrospinning composite nanofiber to capture the colon circulating tumor cells (CTCs) from peripheral blood of colorn cancer patients. CTC detection always influences by extensive leukocyte contamination in human blood. We introduced nylon-6, poly acrylic acid (PAA) and poly sulfobetaine methacrylate (PSBMA) to electrospin as nanofibrous membranes. Nylon-6 is a substrate that can avoid the nanofibers to soluble in aqueous solution. PSBMA is a kind of anti-biofouling polymer to prevent leukocyte absorption. Carboxyl groups of PAA are the media to immobilizate antibody biotin labeling (anti-EpCAM biotin) for CTC capture. The results suggest that the nanofibers composition is optimized to obtain 50/25/25 for Nylon-6/PAA/PSBMA according to the results of antibiofouling and analyte properties.
　　The nanofibers posses to 84.86%, 82.4% and 96.3% of antibiofouling property for fibrinogen, cell endothelial and leukocyte, respectively. Colon cancer cell line (DLD-1, HCT-116 and HT-29), 10 to 100 colon cancer cells, are exploited mixed in 0.4mL medium to perform the capture of CTCs. The results exhibit that detection efficiencies increase linearly upon ca. 65%. Antibiofouling property of PSBMA including is enhanced ca. 18.4% in peripheral blood for CTC detection. Finally, peripheral blood of three colon cancer patients (stage I, II and III) were exploited to examine the property of CTC capture using the naofibers in clinical test. The nanofibers could capture 3.2, 0 and 6.6 per ml of CTCs numbers from the three patients respectively in the clinical test. The current methodologies for CTC capture in peripheral blood always include multiple procedural steps with high cost. Therefore, the nanofiber can possess high potential to capture CTCs clinically.

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