近年來，3D生物列印技術廣泛被許多公司商業化，現有市售機台以擠製型3D生物列印機台為大宗，其多為固定噴頭類型之方式，如需進行更改噴頭類型則須在列印前進行替換與校正之動作，而多數機台在列印進行中無法進行邊列印邊更換噴頭/材料，因此限制了列印材料組合之可能性。因此，本研究擬自行開發具選擇性及操作便利性之多材料列印噴頭模組，進而開發3D生物複合列印系統。
現階段本研究設計之機台具備可在單一列印過程中最多可擠製列印四種不同的生物材料，而四組列印噴頭模組之工作流體皆因氣壓源的過濾相較其他驅動方式較為容易，故採用氣壓作為驅動方式，又其中一組列印噴頭具有光源固化模組以固化含有光起始劑之生物材料。本研究將用於FDM切層軟體與CNC控制器進行整合，以便於相關使用人員能依需求進行設計圖樣及方便列印。又本研究機台最具特色的是，即四組列印噴頭模組可依日後需求進行擴充列印噴頭之種類，其列印材料與列印噴頭皆可依不同需求進行選擇搭配。此外，本研究之機台將導入常見之生物材料進行列印噴頭模組列印測試，以印證設計之機構可順利將材料進行堆疊。

3D Bioprinting Technique has been widely commercialized by many companies in recent years. The present 3D bioprinter is based on Extrusion-based Bioprinting Technique for most, which are mostly fixed printhead types that must be replaced and corrected before printing if needed. However, most of the bioprinters are unable to perform printing and replacement of the printheads/materials during printing, thus limiting the possibility of printing material combinations. Therefore, this study intends to develop multi-material pr inthead module with selectively and ease of operation, and then develop a 3D composite bioprinting system.
At present stage, the bioprinter designed in this study has the ability to bioprint up to four different biomaterials in a single printing process, while the air pressure is used as the working fluid of the bioprinter. One of the printhead has a light source curing module to cure the biomaterial containing the photoinitiator. This study integrate the FDM slicing software with the CNC controller, so that the users can design pattern and facilitate printing according to their needs. The most distinctive feature of bioprinter in this study is that the four sets of printhead modules can expand the type of printhead according to the needs of future. Also, the printing materials and printheads can be selected and matched according to different needs. In addition, the bioprinter of this study will introduce common biological materials for printhead module printing test, in order to verify that the design of the mechanism can smoothly stack the materials.

[1] T. Ozbolat, K. K. Moncal and H. Gudapati (2017). "Evaluation of bioprinter technologies." Additive Manufacturing 13:179-200.
[2] N. Hong, G. H. Yang, J. Lee and G. Kim (2018). "3D bioprinting and its in vivo applications." J Biomed Mater Res B Appl Biomater 106(1):444-459.
[3] Z. Zhang, B. Wang, D. Hui, J. Qiu and S. Wang (2017). "3D bioprinting of soft materials-based regenerative vascular structures and tissues." Composites Part B: Engineering 123:279-291.
[4] Government Research Bulletin: https://www.grb.gov.tw/advq?queryStr=%E8%97%A5%E7%89%A9
[5] %E7%AF%A9%E6%AA%A2&queryType=grb05/
[6] 楊傑名，2015，“台灣首家生物3D 列印，三鼎生技力拼明年登興櫃”，環球生技月刊，2015年10月號.
[7] 宋信文、梁晃千，2003，“建立人類的身體工房-組織工程”，科學發展第362期，6-11.
[8] 杜睿恩，2016，“可光固化poly(geycerol sebacate) acrylate+poly(ε-caprolactone) diacrylate製備新型支架應用於肝組織工程之研究”，國立台灣科技大學機械工程研究所，碩士論文.
[9] 楊婷琪，2002，“組織工程的重要元件-生物分子”，工研院經貿中心生醫組.
[10] M. K. Wlodarczyk-Biegun and A. Del Campo (2017). "3D bioprinting of structural proteins." Biomaterials 134: 180-201.
[11] EnvisioTEC, https://envisiontec.com/
[12] 捷諾飛，http://regenovo.com/English/index.aspx/
[13] GeSiM, http://gesim-bioinstruments-microfluidics.com/
[14] RegenHU, https://www.regenhu.com/
[15] Organovo, https://organovo.com/
[16] nScrypt, https://www.nscrypt.com/
[17] ALLEVI, https://allevi3d.com/
[18] Regemat3D, http://www.regemat3d.com/
[19] CELLINK, https://cellink.com/
[20] 3Dynamic Systems, http://www.bioprintingsystems.com/
[21] Advanced Solutions, http://lifesciences.solutions/bioassemblybot/
[22] Cyfuse, https://www.cyfusebio.com/en/
[23] KOSMEK, http://www.kosmek.co.jp/chinese/
[24] 竣丞國際有限公司，http://www.jc-heatpipe.com/”
[25] 杜鳳琪，“流體力學”，高立圖書有限公司，2006.
[26] 洪國森，2010，“灰關聯分析於點膠流量控制參數最適化之研究”，國立台灣科技大學自動化及控制研究所，碩士論文.
[27] Polysciences, http://www.polysciences.com/
[28] J. Jang, H. J. Park, S. W. Kim, H. Kim, J. Y. Park, S. J. Na, H. J. Kim, M. N. Park, S. H. Choi, S. H. Park, S. W. Kim, S. M. Kwon, P. J. Kim and D. W. Cho (2017). "3D printed complex tissue construct using stem cell-laden decellularized extracellular matrix bioinks for cardiac repair." Biomaterials 112: 264-274.
[29] Newport, https://www.newport.com/