在大豆油除臭油(SODD)分離過程中,修正索式萃取法是一經濟可行的技術。此法經由使用溶劑回收下,可以得的高回收率的非極性脂質(NPLF)。在NPLF,以角鯊烯和固醇之脂肪酸脂(FASE)為最重要的成分。角鯊烯的回收率可達100%,FASEs的回收率可達94.32%。雖然修正索式萃取法所需成本較低,但是使用大量的矽膠當作SODD吸附劑佔了總成本的75~80%。如果可以將矽膠回收再利用,不僅可以節省多餘成本花費也可以利用在廢水處理。
在使用新鮮矽膠當作SODD吸附劑為條件下,最佳的重量比例是1:3 (SODD:矽膠)。但是,當矽膠再生後,以再生矽膠當作SODD吸附劑條件,相同的重量比例1:3(SODD:矽膠)將會使得游離脂肪酸的回收率大量提升至49.71%,遠大於使用新鮮矽膠當作吸附劑的回收率(<20%)。本研究藉由改變矽膠對SODD的重量比例,發現不同比例和不同時間下,對於角鯊烯和FASE的回收率會有不同的影響。使用新鮮的矽膠, 最佳的重量比例是1:3 (SODD:矽膠),最佳時間是12小時;使用第一次再生的矽膠,最佳的重量比例是1:5 (SODD:矽膠),最佳時間是60小時; 使用第二次再生的矽膠,最佳的重量比例是1:8 (SODD:矽膠),最佳時間是110小時;以上這些條件,可使產物回收率達到最佳化,且使FFAs回收率最小化。

Modified soxhlet extraction (MSE) is an economically viable technique in separation of bioactive compounds from SODD. The amount of solvent required in MSE is small and after extraction of non polar lipid fraction (NPLF) and polar lipid fraction (PLF), the solvent can be recovered and reused in the next extraction step. In the NPLF, squalene and FASEs are the most valuable components. The recovery of squalene and FASEs can reach 100% and 94.32%, respectively. The only hindrance in scaling up MSE is the cost of silica gel. A great deal of silica gel as adsorbent could constitute 75~80% of the total cost in MSE. If silica gel can be regenerated and reused, then the operation cost of MSE can be reduced significantly.
When fresh silica gel was used as adsorbent, the optimal mass ratio of SODD-to-silica gel is 1:3. However, when regenerated silica gel was used as the adsorbent, the same degree of separation as that of using fresh silica gel was not attainable under the same operation conditions. At a mass ratio of SODD-to-silica gel of 1:3, FFAs recovery of 49.71% was obtained which is much higher than that obtained by using fresh silica gel as the adsorbent (20% FFAs recovery). This high recovery of FFAs in NPLF would affect the quality of final product. This study investigated the effect of mass ratio of SODD-to-silica gel and extraction time on the recovery of squalene, FASEs, and FFAs. Using fresh silica gel, the optimal conditions achieved are: mass ratio of SODD to silica gel = 1:3, extraction time = 12 h. By using first regenerated silica gel, the optimal conditions achieved are mass ratio of SODD to silica gel = 1:5, extraction time = 60 h. By using second regenerated silica gel, the optimal conditions are mass ratio of SODD to silica gel = 1:8, extraction time = 110 h. Under those 3 set of optimal conditions corresponding to fresh silica gel, 1st and 2nd regenerated silica gel, the MSE can achieve approximately the same degree of separation; i.e., 100% recovery of squalende and ca. 94% recovery of FASEs in the NPLF while the FFAs recovery in NPLF can be kept to less than 20%.

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