本研究為開發一微型過濾晶片，利用掃流式過濾原理，可用於三種不同尺寸範圍的粒子分離，達成細胞分選之目的，使用商用濾膜將其整合於晶片中，此濾膜擁有成本低廉、易於取得的優點，由於本研究使用之晶片由核孔聚碳酸酯濾膜(Nucleopore Polycarbonate Track-Etch)與聚甲基丙烯酸甲酯基材 (PMMA, Polymethyl methacrylate)組成，在晶片封裝時屬於異質黏合，製程較為複雜，故本研究提出(1)以UV光固化膠黏合製程進行黏合兩種不同的材質，並透過螺旋型流道改善檢體於晶片內流動情況，以降低檢體流動不順的現象；(2)雙層掃流式的結構設計，改善先前實驗第二層過濾效果無法持續提升之問題；(3)循環掃流設計，改善先前實驗因檢體有限，需加入其他溶液，以提升過濾效果問題；(4)以人類全血作為分析濾膜孔徑對於血細胞的分離效率之影響，以及測量細胞的存活率。
在先前實驗結果顯示，使用流道寬4mm深2mm之晶片進行循環雙層掃流，將最終過濾出檢體注回初始檢體，能將過濾效果提升至八成左右。從人類全血測試的實驗結果顯示，最佳結果為使用濾膜孔徑尺寸為3 m和0.8 m，可成功攔截33.06~66.55%白血球，但白血球因數量不足，無法檢測出純度；83.48~86.39%的紅血球，純度為99.9%，並收集四到七成血漿；而由於濾膜孔徑與紅血球細胞大小相近，細胞存活率會略為下降。使用濾膜孔徑尺寸5 m和0.8 m，可成功攔截26.79~64.31%白血球，但白血球因數量不足，無法檢測出純度；59.99~81.95%的紅血球，純度為99.9%，並收集到45.45~60.61%血漿。使用濾膜孔徑尺寸2 m和0.8 m，2 m濾膜可成功攔截84.31~84.53%白血球及45.09~46.63%的紅血球，但由於濾膜孔徑與紅血球相近，易造成溶血，不利後續實驗。

The main purpose of this study is to develop a microfluidic chip, which can separate three different sizes of particles by the principle of cross-flow filtration, and achieve the purpose of cell separating. The filter integrated in the chip is a commercial filter, and the cost is low. The advantages are easy to obtain. However, the material of the filter is different from the chip substrate. It is heterogeneously bonded during chip packaging, and the process is complicated. In this study, two different materials are bonded by UV glue bonding process. The spiral channel improves the flow of the sample in the chip and reduces the phenomenon that the sample flow is not smooth. The double cross-flow filtration structure can make the particles not easily block on the filter, and improve the problems caused by excessive pressure. Circulation design improves the need to add other fluids in previous experiments. Using human whole blood as the analysis of the effect of filter pore size on the separation efficiency of blood cells, and measuring the survival rate of cells. The experimental results show that the use of 4mm wide and 2mm deep chip for double-layer cross-flow, combined with circulating design, can effectively improve the filtration effect, but the separation efficiency can't be increased by more than 80%. Experimental results in blood tests show that the filter pore size is 3 m, 0.8 m, which can successfully intercept 33.06~66.55% white blood cells; 83.48~86.39% of red blood cells, purity 99.9%, 40~72% plasma, but the number of white blood cells is insufficient , The purity can't be detected; Cell survival rate will slight drop. The filter pore size is 5 m, 0.8 m, which can successfully intercept 26.79~64.31% white blood cells; 59.99~81.95% of red blood cells, purity 99.9%, 45~60% plasma. The filter pore size is 2 m, 0.8 m, which can successfully intercept 84% white blood cells and 45~46% red blood cells, but vulnerable to hemolysis.

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