本研究利用掃流式過濾系統，分離中國倉鼠卵巢細胞 (Chinese hamster ovary cell, CHO cell) 細胞液中的細胞與蛋白質。由於進行細胞培養時，會於細胞液中加入如血清蛋白等CHO細胞所需的養分，同時細胞生長時會產生代謝物以及蛋白質，因此為達到分離細胞液中細胞以及蛋白質的目的，必須利用過濾的方法。本論文的過濾實驗選用聚乙烯 (polyethylene, PE) 濾膜，孔洞大小約為7~12 μm，CHO細胞大小約20 μm，因此過濾時細胞可被阻擋，而過濾之濾液為細胞所產生的蛋白質與細胞培養液的養分。過濾膜的效果以及持久性取決於與其物理化學性質，由於本研究所使用的PE膜較為疏水，容易造成蛋白質的沾黏而形成濾餅，降低過濾通量，因此本論文提出改質PE膜為較親水的性質，期能抵抗蛋白質的沾黏，降低濾餅的形成，增加過濾通量，並且有效分離代謝產物，將可應用於生物技術以及製藥產業之分離程序。
本論文所採用的改質方式是利用親水性單體，含胺基之丙胺 (propylamine)、丙烯胺 (allylamine) 與乙二醇衍生物包括乙烯氧化乙烯醚 (ethylene oxide vinyl ether, EO1V) 以及二乙烯氧化乙烯醚 (diethylene oxide vinyl ether, EO2V)，以連續式電漿沉積高分子薄膜，並進行表面分析，以及過濾實驗。分析結果發現，四種不同單體電漿沉積後的PE表面，FTIR與ESCA分析結果，皆確定四種親水性單體經電漿聚合成功沉積於基材表面；以水的接觸角測試，PE濾膜表面親水性皆有所提升；表面型態則是藉掃瞄式電子顯微鏡 (SEM) 的觀察，PE表面有顆粒狀與膜狀的形成；原子力顯微鏡 (AFM) 之結果顯示表面粗糙度皆增加。由過濾結果發現，經電漿聚合丙胺、丙烯胺、EO1V與EO2V之高分子的PE濾膜，過濾量分別提高25 %、37 %、43 %與45 %，且濾膜經過去離子水與氫氧化鈉溶液清洗後回復率皆可達80 %與90 %以上，顯示經過電漿聚合親水性單體後，可以有效延緩通量的下降、並提升過濾量，並且濾膜的回復率高，可再利用性加。

This study used crossed-flow filtration system to separate proteins from the Chinese hamster ovary cell (CHO cell) solution. While cultivated cell, usually add some medium like serum protein causing cell generated metabolite and protein, therefore we had to separate protein by filtration. In this work, the size of CHO cell was about 20 μm and so that we chosen PE membrane which has pore size of about 7~12 μm for filtration experiment. Due to the surface hydrophobicity of PE membrane, the protein adhesion usually resulted in blockage of filtration membrane and reduced filtration flux.
This work modified the surfaces of PE membranes by continuous plasma polymerization of hydrophilic monomers including propylamine, allylamine, ethylene oxide vinyl ether (EO1V), and diethylene oxide vinyl ether (EO2V). The deposition of plasma polymers were confirmed by surface characterizations by water contact angle measurements, FTIR, ESCA, SEM analyses and filtration tests. The results showed that the surface hydrophilicity and roughness increased when the PE surfaces were deposited with plasma polymer thin films. For the results of filtration tests, the filtration flux increased 25 %, 37 %, 43 % and 45 % on the modified PE membranes coated with propylamine, allylamine, EO1V and EO2V respectively. Moreover, the flux was recovered to 80 % and 90 % after washing with DI water and NaOH solution, indicating good reusability for the modified membranes.

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