雙相磷酸鈣 (BCP) 主要由高穩定性的氫氧基磷灰石 (HA) 和易於降解的β-磷酸三鈣 (β-TCP) 兩相依不同比例組合而成。本實驗利用高分子輔助法製作BCP，藉由改變煅燒溫度及添加PEG控制物性，其物理性質包括表面型態、結晶度、組成、比表面積及鈣離子釋放行為，並使用SEM、FTIR、XRD、ICP及BET進行檢測與分析。
實驗結果顯示，未加入PEG所製成之BCP會較易出現晶粒聚集，且組成中β-TCP比例會隨著煅燒溫度上升而大幅增加。而添加PEG的組別晶粒較為分散，且在高溫煅燒下仍可保有較多根據在PBS緩衝溶液中BCP的鈣離子釋放，初期鈣離子釋放速率會隨BCP裡β-TCP比例增加而上升，但後期鈣離子釋放平衡濃度則是隨之下降。
體外實驗指出，7F2骨母細胞活性及鹼性磷酸酶分泌皆隨著BCP中β-TCP比例增加而提升。這是因為β-TCP可釋放適量的胞外鈣離子濃度，細胞處於該濃度下可促進其活性及初期骨分化。

Biphasic calcium phosphate (BCP) is composed of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) with different ratios. In this study, BCP is fabricated by novel polymer-assisted method. The physical properties were controlled by varing calcination temperature and by adding PEG. The morphologies, compositions, crystallinity, calcium release and specific surface area of BCP were then analyzed by SEM, FTIR, XRD, ICP and BET.
Without adding PEG, particle aggregation and β-TCP amount increased with calcinations temperature. This was because at high temperature, crystallites was be tended to be aggregated and phase transition from HA to β-TCP was also occurred.
With the addition of PEG, particle dispersed with less aggregation. Although phase transition still happened, HA phase was retained more at high calcinations temperature. This was because PEG would disturb the crystallite aggregation and phase transformation.
From in vitro release, the releasing rate in the beginning was increased but steady concentration of released calcium from BCP was decreased with the amount of β-TCP. Thus, the calcium releasing would be decreased with calcination temperature.
The in vitro results indicated that both of cell viability and ALPase expression increased with β-TCP amounts in BCP. This was because that β-TCP released suitable extracelluar calcium ions to enhance cell viability and osteogenic differentiation. On the contrary, the specific surface area and crystallinity were not so effective on cultured osteoblasts.

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