快速、準確地檢測病原菌對於有效的疾病診斷和管理至 關重要。 這項研究的重點是通過利用環介導等溫擴增 (LAMP) 的力量與微流體紙基分析裝置 (μPAD) 和閉環溫度傳感器相 結合，徹底改變 DNA 檢測。 具體來說，我們的目標是提高即 時護理 (POC) 設備的效率，以準確檢測兩種臨床上重要的細 菌:銅綠假單胞菌和金黃色葡萄球菌。
為了實現這一目標，μPAD 採用先進的 3D 打印技術精 心設計和製造，包含流體源、流體目的地和流體驅動通道的專 用區域。 LAMP 反應在 62°C 的恆溫下進行，由溫度傳感器、 電源、加熱板、銅板和 K 型熱電偶組成的閉環溫度控制系統 精心控制。
通過目視檢查 HNB 彩色染料促進的顏色變化來驗證擴增 結果。 陽性樣本呈現出獨特的淺藍色，而陰性樣本呈現出紫 色調。 使用 MATLAB 軟件進行的進一步定量分析證實了擴增 成功，陽性樣本的譜數約為 350-360，陰性樣本的譜數約為 170。
這種創新方法通過提供可靠、省時且用戶友好的解決方 案，為推動 DNA 檢測領域的發展帶來了巨大希望，特別是在 資源有限的環境中。 這項研究的結果有助於開發高效的 POC 設備，並在醫療保健診斷、環境監測等領域具有潛在的應用。

The rapid and precise identification of harmful bacteria is crucial for effective disease diagnosis and management. This study focuses on revolutionizing DNA detection by harnessing the power of Loop-Mediated Isothermal Amplification (LAMP) in conjunction with microfluidic paper-based analytical devices (μPADs) and closed-loop temperature sensors. Specifically, we aim to enhance the efficiency of point-of-care (POC) devices for accurately detecting two clinically significant bacteria, Pseudomonas aeruginosa and Staphylococcus aureus.
To achieve this, μPADs were meticulously designed and fabricated using advanced 3D printing technology, incorporating dedicated zones for fluid source, fluid destination, and fluid-driven channels. The LAMP process was conducted at 62°C, which was a constant temperature, meticulously controlled by a closed-loop temperature control system comprising a temperature sensor, power supply, heat plate, copper plate, and a type K thermocouple.
Validation of the amplification results was achieved through visual examination of color changes facilitated by the HNB color dye. Positive samples displayed a distinctive light blue color, while negative samples exhibited a purple hue. Further quantitative analysis using MATLAB software confirmed successful amplification, with positive samples demonstrating HSV value around 350-360 and negative samples yielding approximately 170.
This innovative approach holds immense promise for advancing the field of DNA detection, particularly in resource-limited settings, by providing a reliable, time-efficient, and user-friendly solution. The outcomes of this study contribute to the development of highly efficient POC devices with potential applications in healthcare diagnostics, environmental surveillance, and beyond.

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