在生產結構脂質，特別是結構三酸甘油酯時甚少使用像自由脂肪酸與甘油酯化這種從下而上之反應。 雖然有研究報導過自由脂肪酸與甘油酯化，這些多半著眼於合成部分甘油酯或結構脂質之前驅物，而並未證明其真正用於生產結構脂質。通常由上而下之反應，例如新種或特殊油、純三酸甘油酯、特殊脂肪酸以及溶於溶劑中甘油之間酯化常被用來合成時尚新穎的結構脂質。本研究發展一無溶劑之製程將甘油與脂肪酸 ( 癸酸、月桂酸、油酸、棕梠酸) 行酯化反應。經數次測試後，發展出一使用一階段或二階段之靈活程序；後者包括一低溫酯化步驟接著一高溫酯化步驟可以生產相當量之結構脂質以及其前驅物。低限度修正包括反應物加料、一鍋法和等溫方案等用以改善產物組成及產率。結果以100克不含甘油脂產物為基準，可得二酸甘油酯 > 40 克、 中長鏈三酸甘油酯 > 20 克、富含油酸及棕梠酸結構三酸甘油酯 > 40 克。根據此程序，本研究也證實使用像是脂肪酸餾出物之工業副產物之潛力。

Processes based on bottom-up reactions, e.g. esterification of free fatty acids with glycerol, are rarely explored in the production of structured lipids, especially structured triglycerides. Although several cases of esterification of free fatty acids and glycerol have been reported, most of these focused on the synthesis of partial glycerides or precursors for other structured lipids without demonstrating their actual use in structured lipid production. Often, top-down reactions such as inter- and trans-esterification, utilizing novel or specialty oils, pure triglycerides, unique fatty acids, and glycerol dissolved in solvents are employed to synthesize “trendy” or new types of structured lipids. In this study, a process, which is based on solvent-free esterification of free fatty acids and glycerol, is developed using a model reaction system composed of one or more of the following acids with glycerol: capric acid, lauric acid, oleic acid, and palmitic acid. As a result of several tests with various strategies, a flexible process operated either in one- or two-steps, the latter consisting of a low-temperature esterification step followed by a high-temperature esterification step, was established to yield SL and their precursors in substantial amounts. Minimalist modification schemes or techniques, including reactant dosing, one-pot schemes, and isothermal regimes were implemented to improve product composition and process productivity. From the process, diglycerides of > 40 g, medium-long-chain triglycerides of > 20 g, and oleic acid-and-palmitic acid-rich structured triglycerides of > 40 g all based on 100 g of the respective glycerol-free products can be obtained. Furthermore, the potential of utilizing industrial by-products such as fatty acid distillates had been demonstrated through a modification of the baseline process.

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