本研究以靜電紡絲技術製備聚丙烯腈高分子（Polyacrylonitrile, PAN）奈米纖維，先透過氫氧化鈉將奈米纖維表面鹼催化改質使具有羧酸官能基（-COOH），再利用逐層組裝技術（Layer-by-Layer）將兩種不同電性之聚電解質：聚丙烯氯化銨（Poly(allylamine hydrochlorid, PAH)和聚丙烯酸（Poly(acrylic acid), PAA）交互沉積至奈米纖維表面。
本研究利用不同電性之模式藥物，正電性的亞甲基藍（Methylene blue ,MB）及負電性的肝素（Heparin）隨著聚電解質裝載至奈米纖維表面，並透過藥物裝載－釋放實驗（Drug Loading-Release）評估奈米纖維於藥物傳遞之應用潛力，且可藉由調整環境pH值達到藥物控釋之功能。
此外，利用雷射共軛焦顯微鏡（Laser Scanning Confocal Microscope, LSCM）觀察Fluorescein (FITC)-conjugated AffiniPure Goat Anti-Rabbit IgG抗體對於逐層組裝聚電解質奈米纖維之抗沾黏程度，發現聚電解質奈米纖維抗體不利沾黏於奈米纖維上，展現出抗生物沾黏（Anti-biofouling）之特性。

In this work, Polyacrylonitrile (PAN) nanofiber were fabricated by using the electrospinning technique, and the surface was modified with carboxylic acid groups. We deposit two oppositely charged polyelectrolytes: poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) bilayers on the PAN nanofiber by Layer-by-Layer technology.
Furthermore we used two oppositely charged drug: methylene blue (MB) and heparin. These two kinds of drugs were used as the model drug to evaluate the potential application of the nanofibers for drug delivery. The release of MB and heparin was controlled in a phosphate buffered saline (PBS) solution by changing the pH of the solution. The controlled release of drugs from electrospinning nanofibers have potential applications as drug carriers in biomedical science.
In addition, laser scanning confocal microscope (LSCM) measurements were included to determine the relative amount of antibody that adsorbed to these polyelectrolyte nanofibers to examine the property of anti-biofouling.
These polyelectrolyte fibers, due to their simple fabrication and the controlled release property, can be used for drug delivery devices and in other potential application.

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