本研究為設計一共聚高分子材料以偵測人血中的循環腫瘤細胞(CTCs)，在檢測的同時屏除白細胞的干擾。基材為聚二甲基矽氧烷(Polydimethylsiloxane，PDMS)，性質安定且具生物相容性，以液態PDMS覆蓋圖案化的矽晶圓加熱固化，依光罩的設計翻印出高表面積的奈米柱型陣列。以此結構在基材表面以原子轉移自由基聚合法製備共聚高分子刷：(Poly(methacrylic acid)，PMAA)、(Poly(sulfobetaine methacrylate)，PSBMA)，PMAA之羧基可接上streptavidin與anti-EpCAM(Biotin)結合以偵測CTCs，而PSBMA的雙離子性可抵抗生物分子沾黏，達到抗沾黏且可抓取特定細胞的目的。
以不同的高分子比例製備共聚高分子刷，測試出在三種比例中PMAA:PSBMA(75:25)對CTCs專一性抓取率達到了最高的61.7%，且具有96%以上的抗白細胞沾黏能力。在仿臨床實驗中可從10至100顆每毫升下偵測到CTCs，並比PMAA表面抓取率高了6%。
在微流道系統中加入抗沾黏功能，使低濃度液體可重複性流通抓取區而不被白細胞沾黏，有望發展為拋棄式(disposable)的快篩晶片。

In this thesis, we introduced random copolymers on nanopillar-array of polydimethylsiloxane (PDMS) elastomer substrate to capture the colon circulating tumor cells(CTCs) from peripheral blood of colon cancer patients. CTCs detection always influenced by extensive leukocyte contamination in human blood. We fabricated random copolymers of Poly(methacrylic acid) (PMAA) and poly(sulfobetaine methacrylate) (PSBMA) on patterned PDMS. Patterned PDMS was a biocompatible and stable substrate with high surface area. PSBMA was a kind of anti-biofouling polymer to prevent leukocyte adhesion. Carboxyl groups of PMAA were the media to immobilize antibody biotin labeling (anti-EpCAM biotin) for CTCs capture. The results suggest the composition ratio of PMAA/PSBMA copolymer is 75/25 according to the results of anti-biofouling and captured rate properties.
The modified PDMS donated 96% of anti-biofouling property for leukocyte and 61.7% of specific captured rate of CTCs. In clinical simulation, specific capture of CTCs from the solution in 0.1mL medium increased by ca. 10.8%.
The current methodologies for CTC capture in peripheral blood included multiple procedural steps with high cost. Therefore, our research had high potential to capture CTCs on low-cost microfluidic systems.

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