二氧化碳為溫室氣體，會造成全球氣溫上升。微藻的成長需要二氧化碳。本研究探討培養微藻，並利用微藻所含的油脂來生產生質柴油。
微藻的生長受許多因素影響，而影響最適化油脂生產的條件有：氮源濃度、二氧化碳濃度、光照強度、pH、 溫度、收集的時間和從微藻萃取油脂的方法等。
以尿素為氮源，可得到高油脂產率。尿素濃度降低可得到較高之油脂產率。在低氮源濃度下,油脂中三酸甘油脂之含量較高。當以空氣及3.23% CO2一起通氣時, 會有最大的油脂產率。油脂產率最適化的培養溫度、 起始pH和光照強度，分別是22℃、pH 6和10000 lux。
由於時間因素，本研究僅探討微藻生長以及產生高油脂含量之最佳條件。有關利用微藻所含油脂生產生質柴油之研究，將留給後續研究人員做探討。

Carbon dioxide is recognized as the most responsible atmospheric pollutant for the global warming. Microalgae needs CO2 for the growing. This research aims to produce biodiesel from lipid in microalgae.
Several growing conditions which can optimize the production of lipid such as nitrogen concentration, CO2 concentration, light intensity, pH, temperature and culture age as well as method of extracting oil from microalgae will be determined.
High lipid production was observed with urea as the nitrogen source. Lipid production increased with the decreasing of urea concentration. Higher triacylglyceride content in the lipid was observed at low nitrogen concentration. Maximum lipid production occurred when the air was supplemented with 3.23% CO2 (0.05 VVM). Optimal culture temperature, initial pH and light intensity for lipid production were 22 oC, pH 6 and 10000 lux, respectively.
Due to limited time available, only conditions for obtaining optimal algae growth and maximal lipid content were explored. The conversion of microalgae lipid into biodiesel will be left for future investigations.

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