造紙術被稱為中國古代四大發明之一,是由中國東漢的時候,蔡倫改良先前的造紙技術,讓造紙成功率更高,可以使用樹皮、麻等等,這些材料經過許多步驟,就可以實現柔軟且結實的紙張,成本也更低。紙張是一般日常用品,現在有很多東西都是用紙張製作的,像是書本、畫紙、盒子或是裝食物的容器等等。其中宣紙更是在中國古代的時候最常使用的代表,最早是來自安徽省生產,現在宣紙都是用在寫書法或是畫畫的用途上。目前微流控的技術當中,紙微流控技術中,紙張有容易吸水的作用,所以紙微流控有不需要幫浦的優點,讓紙微流控的體積可以更小。我們文化中剛好有宣紙這種紙質材料,希望透過本實驗讓宣紙可以有更多的用處,可以讓全世界看到宣紙。宣紙作為我們基於微流控紙的分析設備研究的一部分。我們使用絲網印刷技術通過低成本製造工藝實現高度靈敏的反應,因為它操作簡單且價格低廉,同時具有高度可擴展性的潛力。我們在絲網印刷技術嘗試使用其他疏水劑,同時應具備容易取得,還有操作簡單、價格便宜等優點。

Papermaking is known as one of the four great inventions in ancient China. During the Eastern Han Dynasty in China, Cai Lun improved the previous papermaking technology to make the success rate of papermaking higher. Bark, hemp, etc. can be used for these materials after many steps. Achieve soft and strong paper at a lower cost. Paper is a general daily necessity, and now many things are made of paper, such as books, drawing paper, boxes or food containers. Among them, Xuan paper is the most commonly used representative in ancient China. It was first produced in Anhui Province. Now Xuan paper is used for writing calligraphy or painting. Among the current microfluidic technologies, in paper microfluidics, paper has the effect of easily absorbing water, so paper microfluidics has the advantage of not requiring a pump, making the volume of paper microfluidics smaller. We just have Xuan paper in our culture. We hope that through this experiment, Xuan paper can be used more and the world can see Xuan paper. Xuan paper as part of our research on microfluidic paper-based analytical devices. We will use screen printing technology and new hydrophobic agents to achieve a highly sensitive reaction through a low-cost manufacturing process, as it is simple to operate and inexpensive while having the potential to be highly scalable.

[1]. Martinez, A. W., Phillips, S. T., Butte, M. J., & Whitesides, G. M. Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angewandte Chemie (International ed. in English) 2007, 46(8), 1318–1320
[2]. Mabey, David, et al. "Diagnostics for the developing world." Nature Reviews Microbiology 2.3 (2004): 231-240..
[3]. Foret, F., Miniaturization and microfluidics in Liquid Chromatography. (ed. Salvatore, F) 454-455 (Foret, 2013).
[4]. Le, N.N., Phan, H.C., Dang, D.M. et al. Fabrication of Miniaturized Microfluidic Paper-Based Analytical Devices for Sandwich Enzyme-Linked Immunosorbent Assays using INKJET Printing. Appl Biochem Microbiol 2021. 57, 257–261
[5]. Zhang, X., Zhi, H., Zhu, M., Wang, F., Meng, Hu., & Feng, L. Electrochemical/visual dual-readout aptasensor for Ochratoxin A detection integrated into a miniaturized paper-based analytical device. Biosensors and Bioelectronics 2021, 180, 113146.
[6]. Tyas, A. A., Raeni, S. F., Sakti, S. P., & Sabarudin, A. Recent Advances of Hepatitis B Detection towards Paper-Based Analytical Devices. The Scientific Journal 2021.
[7]. Ilacas, G. & Gomez, F. A. Microfluidic Paper-based Analytical Devices (μPADs): Miniaturization and Enzyme Storage Studies. Anal Sci. 2019. 35(4), 379-384.
[8]. Shishov, A., Trufanov, I., Nechaeva, D., & Bulatov, A. A reversed-phase air-assisted dispersive liquid-liquid microextraction coupled with colorimetric paper-based analytical device for the determination of glycerol, calcium and magnesium in biodiesel samples. Microchemical Journal. 2019. 150, 104134.
[9]. Martinez, A. W., Phillips, S. T., Wiley, B. J., Gupta, M., & Whitesides, G. M. FLASH: A rapid method for prototyping paper-based microfluidic devices. Lab on a chip 2008, 8(12), 2146–2150.
[10]. Chitnis, G., Ding, Z., Chang, C. L., Savran, C. A., & Ziaie, B. Laser-treated hydrophobic paper: an inexpensive microfluidic platform. Lab on a Chip 2008, 11(6), 1161-1165.
[11]. Amin. R. et al. Pushing the limits of spatial assay resolution for paper-based microfluidics using low-cost and high-throughput pen plotter approach. Micromachines 2020, 11(6), 611.
[12]. Nie, J. et al. Low-cost fabrication of paper-based microfluidic devices by one-step plotting. Analytical chemistry 2012, 84(15), 6331–6335.
[13]. Li, X., Tian, J., Nguyen, T., & Shen, W. Paper-based microfluidic devices by plasma treatment Analytical chemistry 2008, 80(23), 9131–9134.
[14]. Curto, V. F. et al. Fast prototyping of paper-based microfluidic devices by contact stamping using indelible ink. RSC Adv. 2013, 3, 18811-18816.
[15]. Dixon, C., Lamanna, J., & Wheeler, A. R. Printed microfluidics. Adv. Funct. Mater. 2017, 27 (11).
[16]. Maejima, K., Tomikawa, S., Suzuki, K., & Citterio, D. Inkjet printing an integrated and green chemical approach to microfluidic paper-based analytical devices RSC Advances. 2013, 3, 9258-9263.
[17]. Abe, K., Suzuki, K., & Citterio, D. Inkjet-printed microfluidic multianalyte chemical sensing paper. Analytical chemistry 2008, 80(18), 6928–6934.
[18]. Songjoaren, T., Dungchai, W., Chailapakul, O., & Laiwattanapaisal, W. Novel, simple and low-cost alternative method for fabrication of paper-based microfluidics by wax dipping Talanta 2011, 85(5), 2587-93.
[19]. Strong, B., Schultz, S., Martinez, A., & Martinez, N. Fabrication of Miniaturized Paper-Based Microfluidic Devices (microPADs). Sci Rep. 2019, 9, 7.
[20]. Olkkonen, J., Lehtinen, K., & Erho, T. Flexographically printed fluidic structures in paper. Analytical chemistry 2010, 82(24), 10246–10250.
[21]. Dungchai, W., Chailapakul, O., & Henry, C. S. A low-cost, simple, and rapid fabrication method for paper-based microfluidics using wax screen-printing The Analyst 2010, 136(1), 77–82
[22]. Wang, S. et al. Paper-based chemiluminescence ELISA: lab-on-paper based on chitosan modified paper device and wax-screen-printing. Biosens Bioelectronics 2012, 31(1), 212–218.
[23]. Ma, S., Tang, Y., Liu, J., & Wu, J. Visible paper chip immunoassay for rapid determination of bacteria in water distribution system. Talanta 2014, 120, 135–140.
[24]. Namwong, P., Jarujamrus, P., Amatatongchai, M., & Chairam, S. Fabricating simple wax screen-printing paper-based analytical devices to demonstrate the concept of limiting reagent in acid–base reactions. Journal of Chemical Education 2018, 95(2), 305-309.
[25]. Jarujamrus, P. et al. Screen-printed microfluidic paper-based analytical device (μPAD) as a barcode sensor for magnesium detection using rubber latex waste as a novel hydrophobic reagent. Anal Chim Acta 2019, 1082, 66–77.
[26]. Mohammadi, M. et al. An instrument-free, screen-printed paper microfluidic device that enables bio and chemical sensing. Analyst 2015, 140, 6493-6499.
[27]. Juang, Y. J., Li, W. S., & Chen, P. S. Fabrication of microfluidic paper-based analytical devices by filtration-assisted screen-printing. J. Taiwan. Inst.Chem. Eng 2017. 80, 71-75.
[28]. Sun, J. Y., Cheng, C. M., & Liao, Y. C. Screen printed paper-based diagnostic devices with polymeric inks. Anal Sci. 2015, 31(3), 145–151.
[29]. Sameenoi, Y., Nongkai, P., Nouanthavong, S., Henry, C. S., Nacapricha, D. One-step polymer screen-printing for microfluidic paper-based analytical device (μPAD) fabrication. Analyst 2014, 139, 6580-6588.
[30]. Sigma-Aldrich 2021, Whatman® qualitative filter paper, Grade 4. Accessed on 27 April 2021. Retrieved from
[31]. Sigma-Aldrich 2021, Whatman® qualitative filter paper, Grade 1. Accessed on 27 April 2021. Retrieved from Tsai, F. W., & van der Reyden, D. Analysis of modern Chinese paper and treatment of a Chinese woodblock print Pap Conserv. 1997, 21(1), 48-62.
[32]. Luo, Y., Cigić, I.K., Wei, Q., & Strlic, M. Characterisation and durability of contemporary unsized Xuan paper. Cellulose 2021. 28, 1011–1023.
[33]. Zhou, Y. et al. Redefining Chinese calligraphy rice paper: an economical and cytocompatible substrate for cell biological assays. Rsc Adv 2017. 7, 41017-41023.
[34]. Shoon, D. D. Nail enhancement product chemistry in Milady’s Nail Structure & Product Chemistry. (ed. Downs, A) 55-56 (Shoon, 1996).
[35]. Foster, C. W., Metters, J. P., & Banks, C. E. Ultra flexible paper based electrochemical sensors: effect of mechanical contortion upon electrochemical performance Electroanalysis 2013, 25(10), 2275.
[36]. Satarpai, T., & Siripinyanond, A. Alternative Patterning Methods for Paper-based Analytical Devices Using Nail Polish as a Hydrophobic Reagent. Anal Sci. 2018, 34(5), 605–612.
[37]. Bala, R., & Braun, K. M. (2003). Color-to-grayscale conversion to maintain discriminability. Color Imaging IX: Processing, Hardcopy, and Applications, 5293(1), 196. https://doi.org/10.1117/12.532192
[38]. Bachmannm, J., Wooche, S. K., Goebel, M. O., Kirkham, M. B., Horton R., Extended methodology for determining wetting properties of porous media. Water Resources Research. 2003, 39(12) 1353-1364.