研究生: |
賴侑成 You-Cheng Lai |
---|---|
論文名稱: |
水溶性螢光共軛高分子作為具有氣體響應性的自組裝奈米粒子應用於生物成像 Water-soluble Fluorescent Conjugated Polymers as Self-assembled Nanoparticles with Gas-responsiveness for Biological Imaging |
指導教授: |
鄭智嘉
Chih-Chia Cheng |
口試委員: |
邱文英
Wen-Yen Chiu 謝永堂 Yeong-Tarng Shieh 張雍 Yung Chang 蔡協致 Hsieh-Chih Tsai 戴子安 Chi-An Dai |
學位類別: |
碩士 Master |
系所名稱: |
應用科技學院 - 應用科技研究所 Graduate Institute of Applied Science and Technology |
論文出版年: | 2019 |
畢業學年度: | 107 |
語文別: | 中文 |
論文頁數: | 168 |
中文關鍵詞: | 三級胺基團 、生物影像 、氣體響應性 、水溶性共軛高分子 、聚噻吩 |
外文關鍵詞: | tertiary amine functional group, biological imaging, gas-responsiveness, water-soluble conjugated polymers, polythiophene |
相關次數: | 點閱:549 下載:0 |
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本研究成功合成出具有氣體響應性的水溶性共軛高分子,以疏水性噻吩為主幹作為螢光發光基團,具親水性的側鏈三級胺基團作為氣體敏感性的官能基團,並將氣體響應過後的螢光微胞應用於生物成像中。經過二氧化碳和氮氣鼓泡後微胞在水溶液中展現出奈米球型的型態,並可經由簡單通入二氧化碳和氮氣來回切換其粒徑大小以及螢光性質。而此微胞在體外細胞實驗,pH 7.4, 37 °C的生理條件下,對其正常細胞以及腫瘤細胞表現出極低的毒性。此外,在腫瘤細胞的螢光影像以及流式細胞儀的分析證實,經過二氧化碳鼓泡後的微胞展現出比原始及氮氣鼓泡後的微胞有著更高的細胞攝取效率以及螢光強度。更重要的是,在體內斑馬魚胚胎實驗中,經過氣體響應後的微胞在生物體內具有低毒性以及穩定的螢光成像,證實經由氣體響應後的水溶性共軛高分子具有在生物影像中發展的潛力。
This thesis represents an important discovery that employs a gas-triggered strategy to construct water-soluble conjugated polymers (WSCPs) with hydrophobic polythiophene as a fluorescent chromophore and hydrophilic pendant tertiary amines as a gas-sensitive functional group, enabling the generation of gas-responsive fluorescent micelles for applications in biological imaging. The resulting micelles exhibit a nanospherical morphology, tunable particle size and fluorescent properties, in combination with reversibly switchable fluorescent behavior via simple carbon dioxide (CO2) and nitrogen (N2) bubbling. In vitro cell studies revealed the micelles possessed low cytotoxic effects against normal and tumor cells under physiological conditions (pH 7.4, 37 °C). In addition, fluorescent imaging and flow cytometric analyses demonstrated that CO2-treated micelles showed significantly enhanced cellular uptake and fluorescence intensity in tumor cells compared to pristine and N2-treated micelles. More importantly, in vivo studies confirmed that low toxicity and stable fluorescence images in zebrafish embryos were observed for gas-treated micelles, thereby exemplifying the potential applications and usefulness of gas-responsive WSCPs in biological imaging.
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