本研究以開發新型抗菌傷口敷料為主要目的，透過靜電紡絲系統製備出含有銀離子之聚丙烯腈奈米纖維薄膜，利用伽凡尼氧化還原反應，以抗壞血酸作為還原劑，可得含銀聚丙烯腈纖維薄膜，經氧化還原反應與晶核堆積，得海膽形貌之銀-金雙金屬聚丙烯腈纖維薄膜。
利用場發射掃描式電子顯微鏡與能量色散X射線光譜分析奈米纖維薄膜之表面形貌及成分，銀-金雙金屬為454.60 ± 28.14 nm的顆粒鑲嵌於聚丙烯腈纖維上。傅立葉轉換紅外光譜分析其化學結構，確認薄膜基材聚丙烯腈之腈結構仍保留，其吸收峰值為2239 cm-1。表面積及孔徑分析儀分析材料之比表面積，發現薄膜中鑲嵌有銀-金雙金屬顆粒因其顆粒較大，比表面積為10.958 m2/g，小於含銀聚丙烯腈。抗菌能力分析使用革蘭氏陽性菌金黃色葡萄球菌與革蘭氏陰性菌大腸桿菌進行，採用抑菌圈定性與殺菌率定量測試，顯示銀-金雙金屬聚丙烯腈纖維薄膜有較大之抑菌圈，且殺菌效率也遠高於含銀聚丙烯腈纖維薄膜，五小時可達近100 ± 0 %殺菌率。由實驗結果推測可能原因為銀-金雙金屬聚丙烯腈纖維薄膜具備快速且大量釋放銀離子之能力，提供良好的抗菌效果。
小鼠纖維母細胞進行體外測試，當Ag-Au PAN釋放一小時銀離子濃度達0.553 ± 0.09 ppm，產生顯著細胞毒性，表示銀離子濃度對細胞有顯著的毒性依存關係。在活體動物試驗上，以小黑鼠進行動物創傷實驗，在小鼠背部進行皮膚創傷手術，以敷料Aquacel@Convatec(市售抗菌敷料)、Pure PAN、AgPAN和Ag-Au PAN進行治療，並觀察小黑鼠體重、沾黏情形與傷口癒合情形，實驗結果顯示，實驗組Ag-Au APN在第19天傷口已癒合，與對照組無差異，且傷口床之膠原蛋白緻密度為86.39 ± 1.55 %，與健康組織85.27 ± 2.21 %無顯著差異，結果顯示傷口癒合良好，由以上實驗數據顯示，此一海膽形貌之Ag-Au APN為一具有潛力之傷口敷料。

The main purpose of this study was to develop a new type of antibacterial wound dressing. The polyacrylonitrile (PAN) nanofiber film containing silver ions was prepared by an electrospinning system. Ascorbic acid was as a reducing agent to obtain a polyacrylonitrile fiber film containing silver through step by step reduction method. After the Galvanic reaction of the AgPAN nanofiber film with gold in solution and the accumulation of crystallization, a sea urchin-shaped silver-gold bimetallic polyacrylonitrile fiber film with obtained.
The surface morphology and composition of the nanofiber film were analyzed by field emission scanning electron microscope (FE-SEM) and energy dispersive X-ray spectroscopy (EDS). The silver-gold bimetallic particles were 454.60 ± 28.14 nm decorated on the polyacrylonitrile nonwoven mat. The chemical structure was analyzed by Fourier transform infrared spectroscopy (FTIR) to confirm that the nitrile structure of the PAN film remained, and its characteristic absorptiom peak nitrile is showing an absorption peak at 2239 cm-1. Surface area and pore size distribution analyzer (BET) were need to analyzed the surface area of the film, and found the polyacrylonitrile film decorated with Ag-Au bimetallic particles was 10.958 m2/g，which is than that of smaller AgPAN 13.141 m2/g. The antibacterial ability analysis was carried out using Gram-positive bacteria S. aureus and Gram-negative bacteria E. coli strains for qualitative inhibition zone test and quantative sterilization test. Ag-Au PAN showed larger inhibition zone toward S. aureus and E. coli as compared to AgPAN group. The sterilization percentage reached 100% in five hours, and its antibacterial efficiency was significantly faster than that of AgPAN group.
In vitro, we used mouse fibroblasts cells (3T3 cell) for the cell cytotoxicity test. The results showed as the concentration of silver ions increased 1 hour, there was significant cell toxicity observed by Ag-Au PAN. In vivo animal experiment, C57BL/6JNarl mice were subjected to trauma experiments. A skin surgery was performed on the dorsal area of the mice, and the dressings Aquacel, Pure PAN, AgPAN or Ag-Au PAN were applied to treat the wound. The weight of mice, the adhesion index and healing of the wound area were observed. The wound was healed after 19-days treatment, and the collagen density of wound bed is 86.39 %，which is quite close to that of normal skin dermis layer 85.27 %. After healing, the situation of wound was good.

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