本研究合成八種新型離子液體(Good’s buffer ionic liquid)，其中Good氏緩衝劑：N-二羥乙基甘氨酸(Bicine)、3-(N-嗎林)丙烷磺酸(MOPS)、三羥甲基甲胺基丙磺酸(TAPS)、三(羥甲基)甲基甘氨酸(Tricine)作為陰離子的來源；四丁基胺(TBA)與四丁基鏻(TBP)為陽離子的來源。本研究以檸檬酸鈉為鹽析劑使這些離子液體溶液形成雙水相系統後，進行蛋白質萃取。本研究先以雲點法量測含離子液體水溶液與檸檬酸鈉系統的液液相邊界，並藉此找出富離子液體相：富鹽相體積比約為1:1、1:4、1:8時相邊界的質量組成。再以牛血清蛋白與溶菌酶做為目標物，探討在298.2 K與pH = 7下，富離子液體相與富鹽相體積比為1:1、1:4、1:8時，蛋白質的萃取效率。本研究也利用多種生物物理技術進行萃取所得之蛋白質的穩定性分析，相關分析儀器包括紫外可見光、螢光、傅立葉轉換紅外光譜和動態光散射。

In the present study, we synthesize eight new Good’s buffer ionic liquids(GB-ILs) from Good’s buffers (Bicine, MOPS, TAPS or Tricine) as anion part with tetrabutyl-ammonium (TBA) or tetrabutyl-phosphonium (TBP) as cation part. Sodium citrate was used as a salting-out agent to form the ionic liquid aqueous biphasic systems (IL-ABS) for protein extraction. Firstly the liquid-liquid phase boundary of each IL-ABS was determined experimentally by cloud-point method. We obtain the mass compositions of the systems at the volume ratios of the ionic liquid-rich phase to the salt-rich phase around 1:1, 1:4, and 1:8. Using bovine serum albumin and lysozyme as the target materials, explore the extraction efficiencies at 298.2 K and pH = 7 when the volume ratios of the ionic liquid-rich phase to the salt-rich phase is 1:1, 1:4, and 1:8. In this study, we also used various biophysical techniques, including UV-visible, fluorescence, Fourier transforms infrared spectroscopy (FTIR) and dynamic light scattering (DLS) for analyzing the stability of the extracted proteins.

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