經由基因重組過的大腸桿菌所生產的藍藻蛋白 (multi-L-arginyl-poly-L-aspartate, MAPA也被稱為cyanophycin granule polypeptide, CGP)，以天門冬胺酸 (aspartic acid, Asp) 為主鏈，精胺酸 (arginine, Arg) 及離胺酸 (lysine, Lys) 為側鏈所聚合的多胜肽，是一種非核糖體合成的天然蛋白質，具有生物可降解性、酸鹼應答性及高臨界溶解溫度 (upper critical solution temperature, UCST) 特性的智能高分子。
本研究使用瓊脂糖與非水溶性藍藻蛋白利用Schiff base formation反應機制進行交聯反應，降低高分子的相轉移溫度，探討不同交聯率、不同濃度、不同酸鹼值對霧點的影響以及結合金奈米棒的光熱效應探討。
利用高分子酸鹼應答性包覆抗癌藥物阿黴素 (doxorubicin)，另外結合了擁有光熱效應的金奈米棒，建立以照射近紅外光作為誘發
光熱效應使藥物傳遞系統升溫，改變UCST溫敏性高分子結構釋放阿黴素，最後測試於37 oC下含有血清環境的穩定性。

Multi-L-arginyl-poly-L-aspartate (MAPA), also known as cyanophycin, containing a backbone of polyaspartate with arginine and lysine as side chains, was prepared with recombinant Escherichia coli. MAPA is a biodegradable non-ribosomal polypeptide with pH-responsive and UCST (upper critical solution temperature)-type stimuli-responsive property.
In this study, the phase transition temperature of insoluble MAPA (iMAPA) was modified by crosslinking with agarose by Schiff base formation. This study examined the effects of different crosslinking extent, different polymer concentrations, and different pH values on the cloud point as well as a combined photothermal effect with gold nanorods.
The anti-cancer drug doxorubicin and gold nanorod were encapsulated in polymer by utilizing pH-responses of the crosslinked iMAPA/agarose. NIR irradiation can triggered the photothermal effect of gold nanorod; thus, temperature of drug release system increased. Doxorubicin could be released from polymer because of UCST-type response. Finally, the stability in serum-containing buffer was assessed at 37 oC.

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