本研究開發了一種具單向自驅動性的仿生仙人掌刺(cactus spine, CS)結構之微流道表面增強拉曼（SERS）晶片，可應用於生物分子及環境毒物快速臨場檢測。仙人掌刺可以在沙漠中高效蓄積水氣、快速集水並傳輸至根部，本研究以此為發想製作自驅動感測晶片以簡化微流控動力系統。首先利用黃光微影製程複製出仿仙人掌刺微結構於玻璃基板上。在螢光流速測試中證實，最佳化曝光顯影參數所製作之CS微流道可以在無外加幫浦驅動情況下，可以9.83 mm/s之流速自動傳輸螢光溶液通過微流道流至末端。然後再以熱蒸鍍法將有機共軛分子(α,ω- diperfluorohexylquaterthiophene，DFH-4T)及金奈米粒子沉積於微流道末端之玻璃基板，製作具高電磁熱點之常春藤狀的奈米結構作為SERS檢測區。最佳之8 nm 奈米金與1.6 μm DFH-4T沉積參數之常春藤狀SERS晶片成功檢出食安毒物羅丹明B及蘇丹四號，最低檢測濃度可達0.625 ppm。最後製作了相分離微流體SERS晶片並證實可藉親疏水特性與通道效應分離油水相，再以SERS成功檢出水相中之亞甲藍。本研究結果顯示，此仿生CS微流道晶片具有自驅相分離與SERS快速檢測之雙功能，具快速樣本前處理與臨場檢測之潛力。

In this study, a microfluidic integrated surface-enhanced Raman (SERS) chip with a bioinspired cactus spine with unidirectional transport and self-driving was developed for the rapid on-site detection of biomolecules and environmental toxicants. Cactus spines (CS) can effectively accumulate vapor in the desert, quickly collect water, and transport them to the roots, inspiring the study to fabricate self-driving sensors without microfluidic pump systems. First, the study fabricated biomimetic CS mucrostructure on glass substrates via photolithography. In the fluorescence flow rate test, the results confirmed CS microfluidic chips made by optimizing the exposure and development parameters could automatically transport the fluorescent solution to the channel ends at a flow rate of 9.83 mm/s without external pump drives. Furthermore, organic conjugated molecules (α,ω-diperfluorohexylquaterthiophene, DFH-4T) and gold nanoparticles were deposited via thermal evaporation on the microchannel ends glass as SERS detection area, performing ivy-like nanostructures with high electromagnetic hotspots. Optimized parameters of ivy-like SERS chips with 8 nm Au and 1.6 μm DFH-4T successfully detected two food safety toxicants (rhodamine B and Sudan IV), and limitation detection was as low as 0.625 ppm. Finally, the study developed phase-separation microfluidic SERS chips. The results confirmed that the oil-water phase could be separated by hydrophilic/hydrophobic properties and channel effect, and then successfully detected methylene blue using SERS nanotechnology in the water phase.This study showed that the biomimetic CS microfluidic SERS chips have the dual functions of self-driven phase separation and SERS rapid detection, which have the potential for rapid sample pretreatment and on-site detection.

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