汽車廢氣、廚房油煙、焊接燃燒廢氣、燃燒香菸煙氣及空氣中PM2.5過高時等情況，都會在空氣中產生出活性氧物質(Reactive Oxygen Species, ROS)，對經常暴露在活性氧物質環境下的人造成嚴重的傷害。本論文將以植物中所含之多酚類與金屬過渡離子配位化合物所形成之金屬多酚網絡(Metal-Polyphenol Network, MPN)作為奈米酶，用於去除液體及氣體中之活性氧物質。研究主要選用單寧酸與二價銅離子進行研究，此兩者在水溶液中極易形成配位化合物而沉澱析出，改變水溶液之極性及離子強度，則會產生不同形態之奈米沉澱物，如奈米片狀、奈米顆粒及奈米針狀結構沉澱，此三種型態之奈米沉澱物皆具有類過氧化氫酶之活性，可視為一種奈米酶，其中配位螯合之銅離子為類過氧化氫酶活性來源，三種型態當中奈米針狀結構具有最高之類過氧化氫酶活性，其最大反應速率Vmax為1.07×10-6 M/s，而奈米顆粒則對過氧化氫有較好的親和力其Km值為10.09×10–3 M。在Fenton反應溶液中所產生之氫氧自由基(˙OH)亦可被此奈米酶去除，主要是由其中單寧酸所反應消耗，奈米顆粒型態之奈米酶則顯出較高的去除氫氧自由基活性，其濃度在0.8mg/ml時可去除62% 50mM之氫氧自由基。以燃燒香菸之煙氣測試此奈米酶去除氣體中活性氧物質的能力，發現20mg三種型態之奈米酶皆能去除燃燒一根香菸之煙氣中>75%活性氧物質。奈米酶能成功地分別沉積固定於聚丙烯不織布及活性碳不織布上，在活性碳不織布沉積固定表面濃度為8 mg/cm2時，能去除燃燒一根香菸之煙氣中約39%之活性氧物質。

Vehicle exhaust, kitchen fumes, welding combustion exhaust, burning cigarette smoke, and air with high PM2.5 value all generate reactive oxygen species(ROS) in air that cause serious harm to those who are constantly exposed to the hazards of reactive oxygen species. In this study, Metal-Polyphenol Network (MPN) precipitate generated by mixing polyphenol with and transition metal ions, is utilized as a nanozyme to remove reactive oxygen species in water and air. In this work, tannic acid and copper ion were used as model compounds. The nanozyme precipitate from tannic acid and copper ions mixture with different polarity and ionic strength will generate nanozyme of different morphology such as nanosheets, nanoparticles, and nanoneedles. The catalase activity of these nanozymes was first evaluated. It was found that copper ions in the nanozyme plays the major role as catalase. Nanoneedle structure had the highest catalase activity with maximum reaction velocity Vmax value of 1.07×10-6 M/s, while the nanoparticles have a better affinity for the reactant hydrogen peroxide with a Km value of 10.09×10–3 M. The hydroxyl radicals generated in Fenton reaction could also be effectively removed mainly due to the presence of tannic acid in the nanozymes. Approximately, 62% of hydroxyl radical generated from 50 mM H2O2 could be removed by 0.8mg/ml nanoparticle shaped nanozyme. >75% reactive oxygen species from smoke of one burning cigarettes could be removed by employing 20mg of all different shaped nanozyme. The as-prepared nanozymes could be successfully deposited and fixed on polypropylene non-woven fabric and activated carbon non-woven fabric, respectively. The reactive oxygen species removal rate of 39% was achieved for treating smoke from one buring cigarette by employing an activated carbon non-woven fabric loaded with 8 mg/cm2 nanozyme.

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