本研究使用O2大氣電漿與低溫退火技術，對聚甲丙烯酸甲酯 (Polymethyl methacrylate, PMMA)表面進行改質與鍵合，使PMMA/PMMA於表面形成Si–O–Si共價鍵達成鍵合效果，以改變PMMA鍵合的製程方式，由於以往PMMA使用溶劑黏合法會需要在高溫下進行，但這樣的高溫環境會使得PMMA微流道結構產生變形，故與使用溶劑黏合法鍵合的PMMA進行比較，兩種製程的結果其鍵合強度幾乎相當，達到10 MPa的平均值。接著，使用二氧化碳雷射蝕刻 (CO2 laser etching, CLE)製程技術，刻劃出PMMA/ PMMA構成之3D微流道元件，並於電子顯微鏡底下觀察微流道鍵合的結果，觀察孔位對位連接的完整性。最後，使用微流道裝置並結合拉曼光譜儀，對血液進行檢測，該裝置能夠成功收集到各項血液拉曼數據，且其結果與文獻的結果相符合，成功地觀察出許多的生物訊息。

This research uses O2 atmospheric plasma and low-temperature annealing technique to modify and bond the surface of PMMA. The O2 plasma used to modify the surface to form Si–O–Si covalent bonds on the surface of PMMA/PMMA. The formed Si–O–Si bonds changes the process of PMMA bonding. In the past, the solvent bonding method used for PMMA bonding need to be carried out at high temperature, but such a high temperature environment would deform the PMMA microchannel structure, so the bonding with the solvent bonding method is not appropriate for PMMA microchannel bonding. Comparing with solvent bonding and O2 atmospheric plasma bonding of PMMA, the bonding strength of the two processes is almost the same. The mean bonding strength achieves 10 MPa. Then, using CO2 laser etching (CLE) process with sandblasting technique was used to fabricate the PMMA/PMMA 3D micro-channel components, and the bonded micro-channel was analyzed using an electron microscope, and observe the connection of each hole. Finally, a microfluidic device combined with a Raman spectrometer is used to detect blood. The device can successfully collect various Raman data for blood samples, and the results are consistent with those in the literature, and many biological information have been successfully observed.

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