本論文研究目的為製備出奈米銀多層碳管複合材料，將其應用於修飾辣根過氧化氫酶生物感測器，以感測酚類化合物。在材料製備方面，藉由碳酸氫銨將奈米多層碳管官能基化，並加入硝酸銀及二甲基甲醯胺，將奈米銀還原於奈米碳管表面，並利用傅里葉轉換紅外光譜（FTIR）、場發射掃描式電子顯微鏡（FE-SEM）及X-光繞射（XRD）等儀器進行分析及討論。
在辣根過氧化氫酶（horseradish peroxidase, HRP）生物感測器方面，修飾步驟分別為：氧氣電將處理、甲殼素-奈米銀多層碳管及HRP，利用氧氣電漿清潔電極表面及製備出親水表面，以利後續修飾層之塗佈，第二部分為甲殼素-奈米銀多層碳管層，利用甲殼素將HRP固定於電極表面，並利用奈米銀多層碳管提升偵測靈敏度，最後一層為HRP酵素。製備完成後，將此生物感測器感測酚、4-氯酚及鄰苯二酚，感測酚之偵測靈敏度為2.37 μA/μM cm2（0.75 – 100 μM, R2：0.976）及0.749 μA/μM cm2（100 – 400 μM, R2：0.979）；感測4-氯酚之偵測靈敏度為5.821 μA/μM cm2（10 – 60 μM, R2：0.946）及0.704 μA/μM cm2（60 – 120 μM, R2：0.877）；感測鄰苯二酚之偵測靈敏度為0.837 μA/μM cm2（10 – 180 μM, R2：0.989），並且利用感測酚，進行穩定測試、偵測極限測試、長時間保存測試和干擾物測試。
最後一部分為將此生物感測器結合流動注射分析（flow injection analysis system, FIA）系統，以達到連續感測之目的，利用PDMS為基礎之流動感測系統，結合注射式幫補，成功製備出酚類感測器，其電化學性質為2.77 μA/μM cm2（0.5 – 20 μM, R2：0.974）。

This study is comprised of two parts: the first part focused on the synthesis of the complexes of Ag@carbon nanotubes (Ag@CNTs) and preparation of horseradish peroxidase (HRP) biosensor. Carbon nanotubes (CNTs) have drawn considerable attention in recent years because of their superior properties that include the larger specific surface area and more electrocatalytic activity, which can significantly enhance the amperometric anodic signal. In this study, the incorporation of Ag nanoparticles on CNTs aimed to enhance the electrical conductivity and reduce the contact resistance. Moreover, various analytical tools were employed to verify the physical-chemical and morphology characteristics of the prepared Ag@CNTs. An amperometric biosensor base on HRP and chitosan- Ag@carbon nanotubes (Ch-Ag@CNTs) on screen printed carbon electrode (SPE). The current responses were detected by phenolic compound at applied voltage -50 mV. The results showed that two sensitivity of phenol detection were 2.37 μA/μM cm2 (0.75 - 100 μM, R2:0.976) and 0.749 μA/μM cm2 (100 - 400 μM, R2:0.979) and limitation of detection was 0.375 μM. It retained 80% of its initial current response after 30 days.
For the second part, a custom-made flow channel was applied which mainly plays the role of minimize the required components for reactions and further facilitated the integration, automation, and parallelization for the designated biochemical processes. In addition, the prepared Ch-Ag@CNTs in the first part and HRP were incorporated on SPE for the detection of phenol, which was further combined with a custom-made flow channel, to form “Flow Injection Analysis” (FIA). The FIA designed in this study allows the manipulation of small fluid volume, from micro- down to pico- liter, with exceptional accuracy. In summary, the proposed FIA would have the advantages including low price, rapid response time, high accuracy, and smaller reaction volume that can reduce the thickness of the diffusion layer and effectively convey electronic signals between solid-liquid phases when compared with the conventional system, and could be utilized for a wider range of applications. The results showed that the sensitivity of phenol detection were 2.77 μA/μM cm2 (0.5 - 20 μM, R2:0.974).

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