生醫病理的相關研究為現代醫學中重要的技術，從植物組織、動物組織、人體組織或是一些特殊的應用，許多應用都必須靠生醫組織玻片來觀察。早期此項技術都是由傳統的顯微鏡來做病理，或其它特殊研究的觀察，由於醫學研究發展迅速，醫療體系已朝向追求快速、方便、衛生及安全的需求。生醫組織玻片的掃描技術為將一實體的組織玻片，透過顯微影像系統的擷取，轉換成一個全景數位影像的虛擬玻片，進而能在電腦上完成更多的工作。本研究中以組織玻片與薄層細胞抹片（如子宮頸抹片）為例，探討生醫組織玻片掃描設備的顯微影像自動對焦與全景影像縫合等方法，以跨領域方式整合光機電與生物醫學等關鍵性技術，開發生醫組織玻片的掃描雛型設備。

The biomedical pathology is a significant technique in modern medicine, many cell functions must rely on biomedical slides to be observed such as plant tissue, animal tissue, and human tissue. This technique was manipulated with traditional microscope in the past. As the rapid development of medical research, medical system has been tending to pursue quicker, easier, healthier and safer techniques. Biomedical slide scanning technology can convert the slide into a digital panoramic virtual image through the image acquiring systems, which let us be able to do more work on the computer. In this study, we take tissue slice and thin layer cytological smear (Pap smear) as an example to investigate the methods of the autofocusing and panorama stitching, and to development of a prototype biomedical slide scanning system.

[1]Katharina, G. K., Dieter, G. and Michael, J. M., “Virtual Slide: High-quality demand, physical limitations, and affordability,” Human Pathology, Vol. 34, pp. 968-974 (2003)
[2]Treanor, D., “Virtual slides: an introduction,” Diagnostic Histopathology, Vol. 15, No. 2, pp. 99-103 (2009)
[3]Huisman, A., Looijen, A., Steven, M. and Paul, J., “Creation of a fully digital pathology slide archive by high-volume tissue slide scanning,” Human Pathology, Vol. 41, pp. 751-757 (2010)
[4]黃閔照、陳鴻生，「子宮頸抹片篩檢輔助技術之新進展」，台灣醫學，第5券，第2期（2001）。
[5]台灣儀器行，MIRAX SCAN規格，http://www.ticgroup.com.tw/menu/news_center/ new_products/Mirax/mirax.asp（2010）
[6]元利儀器，DotSlide規格，http://www.yuanyu.com.tw/yuanli/Products3_6.asp? Unique_ID=45（2010）
[7]Aperio, “Precise motion control contributes to virtual microscopy slide scanner,” Medical Design Technology, Vol. 10, No. 5, pp. 31-32 (2006)
[8]Firestone, L., Cook, K., Gulp, K., Talsania, N. and Preston, K. J., “Comparison of autofocus methods for automated microscopy,” Cytometry, Vol. 12, pp. 195-206 (1991)
[9]丘前恕，「顯微照像的快速自動對焦技術」，碩士論文，國立中央大學，桃園 (2008)。
[10]Yeo, T. T. E., Ong, S. H., Jayasooriah, and Sinniah, R., “Autofocusing for tissue microscopy,” Image and Vision Computing, Vol. 11 No. 10 pp. 629-639 (1993)
[11]Mea, V. D., Viel, F. and Beltrami, C. A., “A pixel-based autofocusing technique for digital histologic and cytologic slides,” Computerized Medical Imaging and Graphics, Vol. 29, pp. 333-341 (2005)
[12]Li, H., Manjunath, B. S. and Mitra, S. K., “Multisensor image fusion using the wavelet transform,” Graphical Models and Image Processing, Vol. 57, No. 3, pp. 235-245 (1994)
[13]Forster, B., Van, D. V. D., Berent, J., Sage, D. and Unser, M., “Complex wavelets for extended depth of field: A new method for the fusion of multichannel microscopy images,” Microscopy Research and Technique, Vol. 65, pp. 33-42 (2004)
[14]Chen, C. Y., “Image stitching comparisons and new techniques,” Computer Science Department of the University of Auckland, (1998)
[15]Kim, D. H., Yoon, Y. I., and Choi, J. S., “An efficient method to build panoramic image mosaics,” Pattern Recognition Letters, Vol. 24, pp. 2421-2429 (2003)
[16]Zomet, A., Levin, A., Peleg, S., and Weiss, Y., “Seamless image stitching by minimizing false edges,” IEEE Trans. Image Processing, Vol. No. pp. (2006)
[17]Efros, A. A., and Freeman, W. T., “Image quilting for texture synthesis and transfer,” International Conference on Computer Graphics and Interactive Techniques, pp.341-346, (2001)
[18]廖子揚，「影像縫合技術及其應用」，碩士論文，國立交通大學，新竹 (2008)。
[19]Soenksen, D. G., “Fully automatic rapid microscope slide scanner,” US Patent, 6711283, 2004.
[20]Grandall, G. J., “System and method for data management in a linear-array-based microscope slide scanner,” US Patent, 7428324, 2008.
[21]Eguíluz, C., Viguera, E., Millán, L. and Pérez, J., “Multitissue array review: A chronological description of tissue array techniques, applications and procedures,” Pathology-Research and Practice, Vol. 202, pp.561-568 (2003)
[22]許為捷，「組織陣列全自動化設備設計與研製」，碩士論文，國立台灣科技大學，台北 (2009)。
[23]台北榮民總醫院婦產科，子宮頸抹片製作，http://wd.vghtpe.gov.tw/obgy/site. jsp?id=2347（2010）
[24]Song, L. H., Goh, E. S. T., Phang, L. C., Poh, W. T., Tay, S. K., “Technical aspect of ThinPrep,” Singapore Med, Vol. 12, pp. 575-578 (2000)
[25]Kuo, W. M., Chuang, S. F., Nian, C. Y., Tarng, Y. S., “Precision nano-alignment system using machine vision with motion controlled by piezoelectric motor,” Mechatronics, Vol. 18, pp. 21-34 (2008)
[26]Nain, C. Y., Trang, Y. S., “An auto-alignment vision system with three-axis motion control mechanism,” International of Advanced Manufacturing Technology, Vol. 26, pp. 1121-1131 (2005)
[27]Gonzalez, R. C. and Woods, R. E., Digital image processing, Prentice Hall Second Edition, pp.75-141 (2002)
[28]Strang, G., “The discrete cosine transform,” SIAM Review, Vol. 41, pp. 135-147 (1999)
[29]鐘國亮，影像處理與電腦視覺，東華書局，台北，第96-102頁 (2006)。
[30]Santos, A., Solorazno, O. D.,Vaouero J. J., Pena J. M., Malpica N. and Pozo D., “Evaluation of autofocus funtions in molecular cytogenetic analysis,” Journal of Microscopy, Vol. 188, pp. 246-272 (1997)
[31]Vollath, D., “The influence of the scene parameters and of noise on the behaviour of automatic focusing algorithms,” Journal of Microscopy, Vol. 151, 133-146 (1988)
[32]He, J., Zhou, R., and Hong, Z., “Modified fast climbing search auto-focus algorithm with adaptive step size searching technique for digital camera,” IEEE Trans. on Consumer Electronics, Vol. 49, pp. 257-262 (2003)
[33]洪明勝，「掃描攝影機開發生物組織玻片掃描設備之研製」，碩士論文，國立台灣科技大學，台北 (2010)。
[34]Liao, C. W., Yu, J. H., Tarng, Y. S., “On-line full scan inspection of particle size and shape using digital image processing,” Particuology, Vol. 8, No. 3, pp. 286-292, (2010)
[35]Zhang, Z. and Blum, R. S., “A categorization of multiscale-decomposition based image fusion schemes with a performance study for a digital camera application,” Proceedings of the IEEE, Vol. 87, pp. 1315-1326 (1999)
[36]Yoo, J. C., Han, T. H., “Fast normalized cross-correlation,” Circuits Syst Signal Process, Vol. 28 pp. 819-843 (2009)
[37]廖彥法，「立體影像於物件幾何重建之研究」，碩士論文，國立成功大學，台南 (2005)。