本實驗大致可分為四部份，第一部分是製備二氧化矽中空球，並利用化學還原法製Fe/SiO2複合中空球，採用一步法在外層披覆SiO2，粒徑約200±100nm。
第二部份是利用二氧化矽實心球採用化學還原法製備Fe/SiO2複合球，分別使用0.1M、0.375M、0.51M氯化鐵溶液製備，加入TEOS披覆一層SiO2，所得粒徑約200±20 nm。磁性微球的分析上，採XRD、EDS、ICP-MS確認金屬鐵的含量和型態，以TEM確認其微結構，SQUID磁滯曲線測其飽和磁性和矯頑磁力。
第三部份研究則是藥物釋放實驗，藥物釋放實驗以FITC螢光染劑做為模擬藥物，測試釋放行為，其結果顯示Fe/SiO2複合球之釋放行為分為兩步驟，SiO2上的FITC染劑快速釋放行為和SiO2不同。
第四部份研究體外實驗，包括生物適應性、Live/dead staining、TEM切片圖、載體與細胞共培養之光學圖。實驗使用L929細胞做為評估，使用WST-8分別做SiO2、Fe/SiO2載體對細胞活性評估，可以得知複合球活性較SiO2實心球低。於Live/Dead stain image 也能夠用肉眼可見的差異證實了載體濃度不同對細胞存活率的影響，與WST-8測試結果相輔相成。在與細胞共培養的光學照片和TEM切片圖則可觀察到細胞與載體的互動，證實細胞的吞噬作用會把約200nm的載體吞吐進細胞內。

This study is divided into four parts. The first part is to prepare hollow Fe/SiO2 composite spheres by using poly(acrylic acid) as a template and TEOS as a precursor for SiO2 hollow spheres, followed by depositing Fe nanoparticles on hollow spheres with a chemical reduction reaction conducting in an emulsion solution. To avoid the oxidation of Fe nanoparticles, the Fe/SiO2 hollow spheres were covered with a thin SiO2 layer. The final diameter of the Fe/SiO2 hollow sphere is 200±20 nm.
The second part is to prepare Fe/SiO2 composite spheres instead of hollow spheres with three different concentrations of FeCl3 solution. The SiO2-protected Fe/SiO2 composite spheres had the diameter of 200±20 nm.The methods to characterize the Fe/SiO2 spheres are XRD、EDS and ICP-MS to confirm the structure and composition of Fe nanoparticles.
Microstructure was characterized by TEM and the magnetic performance was measured by SQUID.
The third part studies the drug release experiment of Fe/SiO2 composite spheres, compared to that for SiO2-only spheres.
The last part is to study the biological compatibility by WST-8 cell viability test、Live/Dead staining、TEM ultrathin section, and optical image from cell co-culture with drug carriers. The cell used in biological compatibility is L929 together with SiO2 spheres and Fe/SiO2 composite spheres. The result showed that the cell viability of Fe/SiO2 composite spheres was lower than that of SiO2 spheres. The similar result also showed in live/dead staining image. From the TEM image, it had observed the composite spheres inside the cell because of the cell endocytosis behavior .

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